布依药野苏麻的二萜类化学成分研究

Study on Chemical Constituents of Diterpenoids from Buyi Medicine Isodon coetsa

目的:对布依药野苏麻的二萜类成分进行研究,为其资源开发利用提供参考。方法:采用硅胶柱、Sehadex LH-20凝胶柱、MCI柱等对野苏麻的95%甲醇提取物进行分离纯化,通过波谱分析(质谱、氢谱、碳谱)技术鉴定所得化合物的结构,并与民间混用苗药“喔嘎良”的活性成分进行比对。结果与结论:从野苏麻中分离鉴定出10个二萜类化合物,分别为细锥香茶菜乙素(化合物Ⅰ)、大锥香茶菜乙素(化合物Ⅱ)、细锥香茶菜甲素(化合物Ⅲ)、Enanderianin N(化合物Ⅳ)、细锥香茶菜丁素(化合物Ⅴ)、大锥香茶菜甲素(化合物Ⅵ)、线纹香茶菜酸(化合物Ⅶ)、Rubesanolide D(化合物Ⅷ)、Excisanin D(化合物Ⅸ)、Excisanin K(化合物Ⅹ),其中化合物Ⅶ、Ⅸ、Ⅹ均为首次从该植物中分离得到;化合物Ⅶ(线纹香茶菜酸)为布依药野苏麻与苗药“喔嘎良”中共同含有的活性成分。

OBJECTIVE:To study the chemical constituents of diterpenoids from Buyi medicine Isodon coetsa,and to provide reference for the development and utilization of the medicinal resources.METHODS:The 95%methanol extract of Buyi medicine I.coetsa were isolated and purified with silica gel column,Sehadex LH-20 gel column and MCI column.The structures of the compounds were obtained by spectral analysis(mass spectrum,hydrogen spectrum and carbon spectrum),and then compared with active components of Miao medicine“Isodon flavidus”.RESULTS&CONCLUSIONS:Ten diterpenoids were obtained from I.coetsa,including rabdocoetsin B(Ⅰ),megathgrin B(Ⅱ),rabdocoetsin A(Ⅲ),enanderianin N(Ⅳ),rabdocoetsin D(Ⅴ),megathyrin A(Ⅵ),lophanic acid(Ⅶ),rubesanolide D(Ⅷ),excisanin D(Ⅸ),excisanin K(Ⅹ).The compoundsⅦ,ⅨandⅩwere isolated from this specie for the first time.CompoundⅦ(lophanic acid)is a common active component in the Buyi medicine I.coetsa and Miao medicine“I.flavidus”.