HPLC法同时测定还少胶囊中4种成分的含量

Simultaneous Determination of 4 Components in Huanshao Capsules by HPLC

目的:建立同时测定还少胶囊中莫诺苷、马钱苷、松果菊苷和毛蕊花糖苷含量的方法。方法:采用高效液相色谱法。色谱柱为中谱红RD-C18,流动相为乙腈-0.1%甲酸水溶液(梯度洗脱),检测波长为240 nm(莫诺苷、马钱苷)、330 nm(松果菊苷、毛蕊花糖苷),流速为1.0 mL/min,柱温为35℃,进样量为10μL。结果:莫诺苷、马钱苷、松果菊苷、毛蕊花糖苷检测质量浓度的线性范围分别为5.29~105.80、4.49~89.88、16.26~325.25、16.31~326.25μg/mL(r均为0.9999);精密度、稳定性(24 h)、重复性、耐用性试验的RSD均小于2.0%;加样回收率分别为94.34%~96.23%(RSD=0.81%,n=6)、97.04%~98.89%(RSD=0.73%,n=6)、96.23%~98.08%(RSD=0.82%,n=6)、95.40%~98.47%(RSD=1.23%,n=6)。11批还少胶囊中上述4种成分的含量分别为0.190~0.704、0.439~0.857、2.723~4.475、0.589~1.035 mg/g。结论:所建方法专属性强、精密度好,可用于还少胶囊中4种成分的含量测定。

OBJECTIVE:To establish a method for simultaneous determination of morroniside,loganin,echinacoside and acteoside in Huanshao capsules.METHODS:HPLC method was adopted.The determination was performed on Zhongpuhong RD-C18 column with mobile phase consisted of acetonitrile-0.1%formic acid solution(gradient elution)at the flow rate of 1.0 mL/min.The detection wavelength was set at 240 nm(morroniside,loganin)and 330 nm(echinacoside,acteoside).The column temperature was set at 35℃,and sample size was 10μL.RESULTS:The linear range were 5.29-105.80μg/mL for morroniside,4.49-89.88 mg/L for loganin,16.26-325.25 mg/L for echinacoside and 16.31-326.25 mg/L for acteoside,r values were 0.9999.RSDs of precision,stability(24 h),reproducibility and durability tests were all lower than 2.0%.The recoveries were 94.34%-96.23%(RSD=0.81%,n=6),97.04%-98.89%(RSD=0.73%,n=6),96.23%-98.08%(RSD=0.82%,n=6),95.40%-98.47%(RSD=1.23%,n=6),respectively.The contents of above 4 components in 11 batches of Huanshao Capsules were 0.190-0.704,0.439-0.857,2.723-4.475 and 0.589-1.035 mg/g,respectively.CONCLUSIONS:Established method is specific,precise and can be used for content determination of 4 components in Huanshao capsules.