冰片配伍黄芪甲苷和三七总皂苷对大鼠脑缺血再灌注损伤神经元的保护作用

Protective Effect of Borneol Combined with AstragalosideⅣand Panax Notoginseng Saponins on Neurons after Cerebral Ischemia/Reperfusion Injury in Rats

目的探讨冰片配伍黄芪甲苷(astragalosideⅣ,ASTⅣ)和三七总皂苷(panax notoginseng saponins,PNS)对大鼠脑缺血再灌注损伤(cerebral ischemia reperfusion injury,CIRI)后神经元的保护作用。方法成年雄性SD大鼠随机分为假手术组、模型组、冰片组、ASTⅣ组、PNS组、ASTⅣ+PNS组、冰片+ASTⅣ+PNS低剂量组、冰片+ASTⅣ+PNS高剂量组、依达拉奉组。依达拉奉组腹腔注射给药,其他组灌胃给药,2次/d。采用线栓法阻断右侧大脑中动脉(middle cerebral artery,MCA),缺血2 h再灌注22 h建立CIRI模型。行神经功能缺损评分评定神经功能;尼氏染色计数大鼠缺血侧海马CA1区细胞尼氏体数量;免疫荧光法检测缺血侧海马CA1区神经丝蛋白200(neurofilament-200,NF-200)表达;Westrn blot法检测αII-血影蛋白(αII-Spectrin,αII SP)表达。结果CIRI后,模型组神经功能缺损评分显著升高(P<0.01),尼氏体计数显著减少(P<0.01),海马CA1区NF-200及αII SP蛋白表达均显著减少(P<0.01)。冰片+ASTⅣ+PNS组可显著降低神经功能缺损评分,增加尼氏体计数量及NF-200和αII SP蛋白表达(P<0.05,P<0.01),冰片+ASTⅣ+PNS配伍的作用强于各药物单用组及ASTⅣ+PNS组。结论冰片、ASTⅣ、PNS具有抑制CIRI后神经元损伤的作用,3种药物配伍效应增强。

Objective To explore the protective effect of borneol combined with AstragalosideⅣand Panax notoginseng saponins on neurons after cerebral ischemia/reperfusion injury(CIRI)in rats.Methods The adult male Sprague-Dawley(SD)rats were randomly divided into a sham operation group,a model group,a borneol group,a AstragalosideⅣ(ASTⅣ)group,a Panax notoginseng saponins(PNS)group,a ASTⅣ+PNS group,a low dose(borneol+ASTⅣ+PNS)group,a high dose(borneol+ASTⅣ+PNS)group and a edaravone group.The edaravone group was intraperitoneally injected and the other groups were administered by intragastric administration,twice a day.The right middle cerebral artery(MCA)was blocked by wire bolt,and the CIRI model was established after ischemia for 2 hours and reperfusion for 22 hours.The neurological function scores were scored and the number of Nissl bodies in the CA1 area of the right hippocampus of rats were observed by Nissl staining.The protein expressions of neurofilament-200(NF-200)in the CA1 area of the right brain hippocampus were detected by immunofluorescence.The expressions ofαII-Spectrin(αII SP)were detected by Western blot.Results After CIRI,the score of neural dysfunction in the model group was significantly increased(P<0.01),and the number of Nissl body decreased significantly(P<0.01).The expressions of NF-200 andαIISP protein in hippocampus CA1 area were significantly reduced(P<0.01).Borneol+AST IV+PNS could significantly reduce the score of nerve function defect,and increased the number of Nissl body,the expression of NF-200 andαII SP protein(P<0.05 and P<0.01).The borneol+AST IV+PNS effect was better than that of single drug and AST IV+PNS.Conclusion Borneol,ASTⅣ,and PNS have anti-neuronal damage after cerebral ischemia reperfusion injury,and the effect of the combination of the three drugs was enhanced.