TCF7L2 rs290481基因多态性与2型糖尿病合并冠心病相关

TCF7L2 rs290481 polymorphisms are correlated to type 2 diabetes combined with coronary heart disease

目的探讨转录因子7类似物(TCF7L2)基因rs290481位点单核苷酸多态性(SNPs)与2型糖尿病合并冠心病的相关性。方法选取泉州地区汉族120例单纯2型糖尿病(T2DM)患者、120例冠心病(CHD)患者、120例2型糖尿病伴发冠心病(T2DM+CHD)患者和120名健康对照者(control)。提取外周血DNA,用聚合酶链式反应(PCR)扩增TCF7L2后测序;用χ2检验法分析不同组别间基因型分布频率的差异;用两分类Logistic回归分析糖尿病合并冠心病的危险因素。结果T2DM组TCF7L2基因rs290481 AA、AG、GG基因型分布频率与对照组、CHD组、T2DM+CHD组比较均有明显差异(P<0.01)。AA基因型和AG/GG基因型之间HbAlc、GLU水平有明显差异(P<0.05)。Logistic回归表明,高血压病史、HbAlc、GlU和TCF7L2基因rs290481位点AG/GG基因型是T2DM合并CHD发生的独立危险因素。结论TCF7L2 rs290481基因位点多态性与T2MD合并CHD相关,AG/GG基因型较AA基因型患者T2MD合并CHD的患病风险较高。

Objective To investigate the correlation between single nucleotide polymorphisms(SNPs)on the rs290481 site of TCF7L2 gene and type 2 diabetes mellitus complicated with coronary heart disease(CHD).Methods Peripheral blood DNA was extracted and TCF7L2 gene was amplified by polymerase chain reaction(PCR)and then sequenced in 120 patients with simple type 2 diabetes(T2DM),120 patients with coronary heart disease(CHD),120 patients with type 2 diabetes combined with coronary(T2DM+CHD)and 120 healthy controls.Chi-square test was used to analyze the difference of genotype frequency in four groups and binary logistic regression was used to analyze the risk factors of diabetes with coronary heart disease.Results The distribution frequency of AA,AG and GG on TCF7L2 and rs290481 SNP sites from T2DM group were significantly different with the controls,CHD group and T2DM plus CHD group respectively(P<0.01).The serum level of HbAlc and GLU,was significantly different between AA and AG/GG genotypes(P<0.05).Logistic regression data demonstrated that history of hypertension,HbAlc,GLU and the rs290481 AG/GG genotype of TCF7L2 were independent risk factorsof diabetes mellitus complicated with CHD.Conclusions The polymorphism of TCF7L2 rs290481 gene is associated with the risk of type 2 diabetes complicated with CHD.AG/GG genotype confer a significantly increased risk of type 2 diabetes mellitus with CHD.