生物玻璃/聚乳酸多孔微球的制备及其作为细胞载体的研究

Bioglass/Polylactic Acid Porous Microspheres:Preparation and Their Application as Cell Microcarriers

具有大孔结构的多孔微球既可以在体外扩增细胞,还可以作为细胞的传输工具,通过注射的方式把细胞输送到需要修复的组织部位。生物玻璃虽然生物活性良好,但难以直接制备成大孔结构的微载体。因此,本研究将生物玻璃(BG)与聚乳酸(PLA)高分子复合,通过复乳法制备了一种含生物玻璃的多孔微球细胞微载体。并通过扫描电镜(SEM)、热重分析(TGA)、电感耦合等离子体发射光谱仪(ICP-OES)等方法研究分析了微球的形貌、组成和离子释放。通过细胞实验,证明细胞可以在微球的多孔结构中粘附和增殖,并且生物玻璃可以促进细胞增殖,在组织工程中具有潜在应用。

Porous microsphere cell microcarrier with macroporous structure can not only amplify cells in vitro,but also serve as cell delivery tools to deliver cells to damaged tissues by injection.Bioglass(BG)is an inorganic material with excellent biological activity,however,it is difficult to directly prepare microcarriers with macroporous structure.Therefore,in this study,a BG/poly-lactic acid(PLA)porous microspheres was prepared by double emulsion method.The morphology and structure of the microsphere were characterized by SEM,and the load of BG in the microsphere was characterized by thermo gravimetric analysis and its ion release was detected by ICP.Cell proliferation experiments showed that cells could adhere and grow on the surface and inside of the microspheres.The results show that the microsphere with interconnected open-pore microstructure is suit for cell adhesion and proliferation.Bioglass can promote cell proliferation in the microspheres and the obtained BG/PLA composite microsphere has great potential applications in tissue engineering.