Copine-8在胃癌组织中的表达与临床病理特征及预后的相关性

Expression of Copine-8 in gastric cancer and its correlations with clinicopathological features and prognosis

目的探讨Copine-8(CPNE8)在胃癌组织中的表达与患者临床病理特征及预后的相关性。方法收集2011年3月—2014年3月于仙桃职业学院附属医院行胃癌根治术并经过病理证实的胃癌组织标本144例,采用免疫组织化学分析CPNE8在胃癌及其配对的癌旁组织中的表达情况,并分析其与胃癌临床病理参数及预后的关系。结果免疫组织化学结果表明,CPNE8阳性表达率在胃癌组织中较癌旁组织中明显升高(63.9%vs.26.4%,χ^2=36.450,P<0.001);CPNE8的表达与胃癌患者的TNM分期及有无淋巴结转移密切相关(χ^2=5.993,P=0.014;χ^2=6.703,P=0.009)。Kaplan-Meier生存分析发现,CPNE8高表达患者总生存时间(OS)和无病生存时间(DFS)均明显短于CPNE8低表达患者(χ^2=23.130,P<0.001;χ^2=21.570,P<0.001)。TNM分期(HR=1.297,95%CI1.018~1.652,P=0.036)、淋巴结转移(HR=1.340,95%CI1.027~1.749,P=0.031)和CPNE8蛋白表达(HR=1.531,95%CI1.208~1.940,P<0.001)是影响胃癌患者OS的独立危险因素;TNM分期(HR=1.280,95%CI1.028~1.593,P=0.027)和CPNE8蛋白表达(HR=6.010,95%CI1.355~26.661,P=0.018)是影响胃癌患者DFS的独立危险因素。结论CPNE8在胃癌组织中显著表达上调,并与患者恶性病理特征密切相关,CPNE8可成为胃癌患者预后评估的标志物之一。

Objective The aim of this study was to investigate the correlation between the expression of copine-8(CPNE8)and clinicopathological characteristics and prognosis of gastric cancer.Methods A total of 144 patients with gastric cancer who underwent radical gastrectomy in the Affiliated Hospital of Xiantao Vocational College and confirmed by pathology from March 2011 to March 2014 were collected.The expression of CPNE8 in gastric cancer and its matched adjacent tissues were analyzed by immunohistochemistry,and its relationship with clinicopathological parameters and prognosis of gastric cancer was analyzed.Results Immunohistochemical results showed that the positive expression rate of CPNE8 in gastric cancer tissues was significantly higher than that in adjacent tissues(63.9%vs.26.4%;χ^2=36.450,P<0.0001).The expression of CPNE8 is closely related to the TNM stage and lymph node metastasis in patients with gastric cancer(χ^2=5.993,P=0.014;χ^2=6.703,P=0.009).Kaplan-Meier survival analysis showed that the overall survival time(OS)and disease-free survival time(DFS)of patients with high expression of CPNE8 were significantly shorter than those with low expression of CPNE8(χ^2=23.130,P<0.001;χ^2=21.570,P<0.001).The TNM stage(HR=1.297,95%CI1.018~1.652,P=0.036),lymph node metastasis(HR=1.340,95%CI1.027~1.749,P=0.031)and the expression of CPNE8 protein(HR=1.531,95%CI1.208~1.940,P<0.001)were independent risk factors influencing OS of gastric cancer patients.The TNM stage(HR=1.280,95%CI1.028~1.593,P=0.027)and the expression of CPNE8 protein(HR=6.010,95%CI1.355~26.661,P=0.018)were independent risk factors for DFS of gastric cancer patients.Conclusion The expression of CPNE8 is significantly up-regulated in gastric cancer tissues,and is closely related to the malignant pathological characteristics of patients.CPNE8 may be one of the prognostic markers for gastric cancer patients.