Omi/HtrA2与Livin对子痫前期及子痫患者胎盘凋亡的影响

The influence of Omi/HtrA2 gene and Livin gene of pregnnant women with preeclampsia or eclampsia on their placenta cell apoptosis

目的:检测子痫前期及子痫患者(观察组)和正常晚期妊娠孕妇(对照组)胎盘组织中Omi/HtrA2基因与凋亡抑制蛋白Livin基因表达,探讨Omi/HtrA2与Livin在子痫前期及子痫胎盘凋亡中的作用。方法:采用免疫组织化学SP法检测60例观察组和20例对照组分娩后胎盘组织中Omi/HtrA2与Livin表达。结果:Omi/HtrA2主要在胎盘组织中滋养细胞及间质细胞胞质中表达,观察组表达水平(150.46±26.59)高于对照组(132.11±18.43),子痫前期重度(161.17±19.45)高于子痫前期(136.79±26.57)(P<0.05);Livin主要在胎盘组织中合体滋养细胞及间质细胞的细胞核中表达,观察组表达水平OD值(116.07±22.40)低于对照组(136.73±20.81)(P<0.01),但子痫前期与子痫前期重度组间比较无差异(P>0.05)。结论:Omi/HtrA2、Livin均参与了胎盘的凋亡,在子痫前期及子痫发生发展中可能有重要作用。

Objective:To detect the expressions of Omi/HtrA2 gene and anti apoptosis protein Livinin gene in placentas of normal women or women with preeclampsia or eclampsia during the third trimester of pregnancy,and to explore the role of Omi/HtrA2 and Livin in placenta cell apoptosis of women with preeclampsia or eclampsia.Methods:Immunohistochemical SP method was used to detect Omi/HtrA2 and Livin expressions in placental tissues after delivery of 60 women in the observation group and 20 normal pregnant women in control group.Results:Omi/HtrA2 was mainly expressed in trophoblast cells and stromal cells cytoplasm of placental tissues.The expression level of Omi/HtrA2 of women in the observation group was 150.46±26.59,which was significant higher than that(132.11±18.43)of women in the control group,and the expression level of Omi/HtrA2 of women with severe preeclampsia was 161.17±19.45,which was significant higher than that(136.79±26.57)of women with preeclampsia(P<0.05).Livin was main expressed in the nuclei of syncytiotrophoblast cells and stromal cells in the placental tissues.OD value of women in the observation group(116.07±22.40)was significant lower than that(136.73±20.81)of women in the control group(P<0.01),but there was no significant different in the Livin expression between women with preeclampsia and women with severe preeclampsia(P>0.05).Conclusion:Omi/HtrA2 and Livin are both involved in placental cells apoptosis and may play an important role in the occurrence and development of preeclampsia.