XRCC1及XPD基因多态性与高海拔地区非小细胞肺癌的关系及其对预后的意义

Relationship between XRCC1 and XPD gene polymorphisms and non-small cell lung cancer in high altitude area and its significance for prognosis

目的:探讨XRCC1及XPD基因多态性与高海拔地区非小细胞肺癌(NSCLC)的关系及其对预后的意义。方法:选取122例高海拔地区非小细胞肺癌患者为研究对象。采集患者外周静脉血,并提取血液标本基因组DNA,应用限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术及基因测序法进行基因型判定。比较XRCC1-194、XRCC1-28及XPD-312位点的基因型分布频率与NSCLC临床特征的关系;比较XRCC1及XPD基因多态性与患者预后的关系。结果:XRCC1-194、XRCC1-280、XPD-312基因多态性在NSCLC不同分化程度及临床分期中,差异均有统计学意义(P<0.05);XRCC1-194位点CC、CT、TT基因型生存曲线无明显差异(P>0.05),XRCC1-280位点AA、GG基因型和XPD-312位点AA、GA、GG位点基因型生存曲线差异有统计学意义(P<0.05)。结论:XRCC1及XPD基因是修复损伤基因,XRCC1-194、XRCC1-280及XPD-312位点不同基因型与高海拔地区NSCLC患者的临床特征有着密切关系,其中XRCC1-280及XPD-312位点不同基因型可作为判断患者预后的标志物。

Objective:To explore the relationship between XRCC1 and XPD gene polymorphism and non-small cell lung cancer(NSCLC)in high altitude area and its significance for prognosis.Methods:122 cases of NSCLC in high altitude areas were selected as the study objects.The peripheral venous blood of patients was collected,and the genomic DNA of blood samples was extracted.The genotypes were determined by PCR-RFLP and gene sequencing.The genotype frequencies of XRCC1-194,XRCC1-280 and XPD-312 were compared with the clinical characteristics of NSCLC.The relationship between XRCC1 and XPD gene polymorphism and prognosis was compared.Results:The XRCC1-194,XRCC1-280,XPD-312 gene polymorphism and NSCLC differentiation degree,and clinical stage were statistically significant(P<0.05).There was no significant difference in survival curves of CC,CT and TT genotypes at XRCC1-194 locus(P>0.05).There were significant differences in survival curves of AA,GG genotypes at XRCC1-280 and AA,GA,GG genotypes at XPD-312(P<0.05).Conclusion:XRCC1 and XPD genes are repair damage genes.Different genotypes of XRCC1-194,XRCC1-280 and XPD-312 loci are closely related to the clinical characteristics of NSCLC patients in high altitude areas,and different genotypes of XRCC1-280 and XPD-312 loci can be used as markers to judge the prognosis of patients.