Spindly和Bub3在口腔鳞状细胞癌中的表达及意义

Expression and clinical significance of Spindly and Bub3 in oral squamous cell carcinoma

目的:探讨Spindly和Bub3在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中的表达水平及意义。方法:选取河北医科大学第四医院2017年3月—2019年3月收治的65例OSCC患者的口腔鳞癌组织及癌旁正常组织,采用RT-PCR检测口腔鳞癌组织及癌旁正常组织中Spindly、Bub3 mRNA的表达水平;对OSCC细胞株进行培养,siRNA转染干扰Spindly、Bub3基因表达后,采用MTT法检测细胞增殖能力,划痕实验检测细胞迁移能力。采用SPSS 22.0软件包对数据进行统计学分析。结果:Spindly和Bub3 mRNA在人口腔鳞癌组织中的表达水平显著高于癌旁组织(P<0.05);Spindly和Bub3 mRNA表达量与T分期、临床分期、淋巴结转移有关(P<0.05);Spindly、Bub3、Spindly/Bub3高表达患者的5年生存率高于低表达患者,且Spindly/Bub3高表达患者的5年生存率低于Spindly、Bub3高表达患者,差异有统计学意义(P<0.05);siRNA-Spindly组的Spindly表达水平低于Spindly阴性对照组、空白对照组,siRNA-Bub3组的Bub3表达水平也低于Bub3阴性对照组、空白对照组,差异具有统计学意义(P<0.05);siRNA-Spindly组细胞在24、48、72、96 h的增殖能力低于Spindly阴性对照组、空白对照组,siRNA-Bub3组细胞在24、48、72、96 h的增殖能力低于Bub3阴性对照组、空白对照组,差异有统计学意义(P<0.05);siRNA-Spindly组细胞在24、48 h的迁移能力低于Spindly阴性对照组、空白对照组,siRNA-Bub3组细胞在24、48 h的迁移能力低于Bub3阴性对照组、空白对照组,差异有统计学意义(P<0.05)。结论:Spindly和Bub3在口腔鳞癌中呈高表达,且与临床、病理分期相关;特异性抑制Spindly和Bub3基因表达,可降低癌细胞的增殖和迁移能力,有望作为治疗口腔鳞癌的靶点之一。

PURPOSE:To investigate the expression and significance of Spindly and Bub3 in oral squamous cell carcinoma(OSCC).METHODS:Sixty-five patients with OSCC admitted to the Fourth Hospital of Hebei Medical University from March 2017 to March 2019 were enrolled.RT-PCR was used to detect the expression of Spindly and Bub3 mRNA in oral squamous cell carcinoma and adjacent normal tissues from the patients.OSCC cell line was cultured.After siRNA transfection interference with the expression of Spindly and Bub3 genes,cell viability was detected by MTT assay,and the cell migration ability was detected by scratch test.Statistical analysis was performed with SPSS 22.0 software package RESULTS:The expression levels of Spindly and Bub3 mRNA in OSCC were significantly higher in adjacent tissues(P<0.05).The expression of Spindly and Bub3 mRNA was related to TNM staging,clinical staging and lymph node metastasis(P<0.05).The 5-year survival rate of patients with high expression of Spindly,Bub3,Spindly/Bub3 were significantly higher than the counterparts with low expression,and the 5-year survival rate of patients with high expression of Spindly/Bub3 was significantly lower than that of patients with high expression of Spindly and Bub3(P<0.05).The expression level of Spindly in siRNA-Spindly group was significantly lower than that in Spindly negative control group and blank control group,and the expression level of Bub3 in siRNA-Bub3 group was also significantly lower than that in Bub3 negative control group and blank control group.The expression level of Spindly in siRNA-Spindly group was significantly lower than that in Spindly negative control group and blank control group,and the expression level of Bub3 in siRNA-Bub3 group was also lower than that in Bub3 negative control group and blank control group.The migration ability of cells in siRNA-Spindly group at 24 and 48 hours was significantly lower than that in Spindly negative control group and blank control group;the migration ability at 24 and 48 hours was significantly lower than that of Bub3 negative control group and blank control group(P<0.05).CONCLUSIONS:Spindly and Bub3 are highly expressed in OSCC.Specific inhibition of Spindly and Bub3 gene expression can reduce the proliferation and migration of cancer cells,which might be used as one of the targets for the treatment of OSCC.