沉默lncRNA TUG1对肾透明细胞癌细胞增殖、凋亡及迁移的影响

Effect of silencing lncRNA TUG1 on proliferation,apoptosis and migration of ccRCC cells

目的:探究沉默lncRNA TUG1对肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)细胞增殖、凋亡及迁移的影响。方法:选用37例ccRCC组织及癌旁组织样本,通过提取样本总RNA并行逆转录及转染,利用qRT-PCR检测lncRNA TUG1在ccRCC组织、细胞系及癌旁组织中的表达,并通过CCK-8法、流式细胞检测法及划痕实验检测沉默lncRNA TUG1对ccRCC细胞A704、A498增殖、凋亡及迁移的影响,采用统计软件进行数据处理,探究lncRNA TUG1在ccRCC中的表达及临床意义。结果:qRT-PCR检测lncRNA TUG1表达情况,结果显示:lncRNA TUG1在ccRCC组织中的表达显著高于癌旁组织,在肾细胞癌细胞系A704和A498中的表达明显高于正常细胞系HK2,差异有统计学意义(P<0.05);CCK-8检测细胞增殖情况,结果显示:A704和A498细胞经转染干扰后细胞增殖活性较对照组显著降低(P<0.05),沉默lncRNA TUG1可抑制细胞增殖;流式细胞仪检测细胞凋亡情况,结果显示:A704和A498细胞凋亡比例较空白对照组明显增多(P<0.05),沉默lncRNA TUG1可促进细胞凋亡;划痕实验检测细胞迁移能力,结果显示:A704和A498细胞迁结论:沉默lncRNA TUG1可诱导ccRCC细胞凋亡,抑制其增殖、迁移,可作为临床研究的新靶点。

Objective:To investigate the effect of silencing lncRNA TUG1 on proliferation,apoptosis and migration of ccRCC cells.Methods:37 cases of ccRCC tissues and adjacent tissue to carcinoma were collected.Total RNA was extracted with reverse transcription and transfection.qRT-PCR detected lncRNA TUG1 expression in ccRCC tissues and cell lines and in normal tissue adjacent to carcinoma,and through the CCK-8 method,flow cytometry assay and scratch test detected the effect of silence lncRNA TUG1 on A704,A498 ccRCC cell proliferation,apoptosis and migration.Results:The expression of lncRNA TUG1 detected by qRT-PCR showed that the expression of lncRNA TUG1 in the ccRCC tissues was significantly higher than that in the adjacent tissues,and the expression of lncRNA TUG1 in the renal cell carcinoma cell lines A704 and A498 was significantly higher than that in the normal cell lines HK2,and the difference was statistically significant(P<0.05).CCK-8 was used to detect cell proliferation,and the results showed that the proliferation activity of A704 and A498 cells after transfection interference was significantly lower than that of the control group(P<0.05),and the silencing of lncRNA TUG1 could inhibit cell proliferation.Cell apoptosis was detected by flow cytometry,and the results showed that the apoptosis ratio of A704 and A498 cells was significantly higher than that of the blank control group(P<0.05),and the silencing of lncRNA TUG1 could promote cell apoptosis.Cell migration was detected by scratch test,and the results showed that the migration ability of A704 and A498 cells was significantly lower than that of the blank control group(P<0.05),and the silencing of lncRNA TUG1 could inhibit cell migration.Conclusion:Silencing lncRNA TUG1 can induce apoptosis of ccRCC cells and inhibit their proliferation and migration,which may be a new target for clinical studies.