脾虚证高乳糖腹泻模型建立和评价

Establishment and Evaluation of High-lactose Diarrhea Model with Pi Deficiency Syndrome

目的建立和评价慢性腹泻并符合脾虚证证侯特点的病证结合动物模型。方法 40只成年Wistar大鼠随机分为正常组(10只)、高乳糖饲料喂养组(高乳糖组,10只)、水环境小平台站立组(小平台组,10只)、高乳糖饲料叠加小平台组(叠加组,10只)。正常组使用正常标准饲料喂养,高乳糖组分别用50%、60%、70%递增高乳糖饲料喂养3周,小平台组是每天在水环境站立9 h,叠加组是将高乳糖组与小平台站立两种处理因素叠加模拟乳糖不耐受慢性腹泻脾虚证模型,观察大鼠一般体征、粪便含水量,测定抓力及血D-木糖水平。结果与正常组比较,高乳糖组大鼠体重下降(P<0.001),进食量增加(P<0.01),大便含水量增加(P<0.01),抓力测定值降低(P<0.05);小平台组体重下降(P<0.001),进食量减少(P<0.05),但无腹泻。叠加组大鼠体重下降(P<0.001),进食量减少(P<0.05),大便含水量增加(P<0.01),抓力测定值降低(P<0.01),肠胀气明显,D-木糖含量下降(P<0.01)。叠加组较高乳糖组体重下降(P<0.001)。小平台组较高乳糖和叠加组血清D-木糖明显升高(P<0.05)。结论梯度增加高乳糖浓度喂养大鼠合并小平台站立可以成功建立乳糖不耐受慢性腹泻脾虚证模型。

Objective To establish and evaluate chronic diarrhea rat model in line with Pi deficiency syndrome(PDS).Methods Forty male adult Wistar rats were randomly divided into four groups:the normal control group(n=10),high lactose diet group(n=10),small platform group(n=10),high lactose diet plus small platform group(superimposed group,n=10).Rats in the normal control group were fed with normal standard diet.Those in the high lactose group were fed with 50%,60%and 70%increased lactose for 3 successive weeks.Rats in the small platform group stood in water environment for 9 h every day.And rats in the superimposed group were treated with two factors of high lactose group and small platform group(to simulate PDS model of lactose intolerance induced chronic diarrhea).General signs,fecal water content,grasp power,and D-xylose level were observed and determined.Results Compared with the normal control group,body weight was reduced(P<0.001),food intake reduced(P<0.01),fecal water content increased(P<0.01),and grasping power decreased(P<0.05)in rats of the high lactose group(P<0.05).Body weight was reduced(P<0.001)and food intake reduced(P<0.05)in rats of the small platform group,but with no diarrhea.In rats of the superimposed group,body weight was reduced(P<0.001),food intake reduced(P<0.05),fecal water content increased(P<0.01),and grasping power decreased(P<0.01),the flatulence was obviously seen,and the D-xylose content decreased(P<0.01).The body weight decreased more significantly in the superimposed group than in the high lactose diet group(P<0.001).The level of D-xylose significantly increased in the small platform group(P<0.05),as compared with the superimposed group and the high lactose group.Conclusion The model of high lactose induced-diarrhea with PDS could be successfully established by feeding high lactose diet with gradient increase concentrations plus standing on the small platform in water environment.