结合光镊与微流控技术的红细胞变形性高通量检测及表征方法

High throughput detection and characterization of red blood cells deformability by combining optical tweezers with microfluidic technique

提出了一种结合光镊技术与微流控芯片的人血红细胞变形性高通量检测方法,用于红细胞变形性的统计学准确表征。首先,利用光阱力与流体粘滞阻力的共同作用实现红细胞的有效拉伸变形;然后,利用图像处理方法对单细胞变形前后的特征参量进行提取,得到面积和周长变形指数;最后,进行统计学分析,并利用平均变形指数参量(、)对红细胞的变形性进行表征。搭建了高通量检测系统,通过大量实验得到了最优实验条件;并利用具有不同变形性的3组样本进行了统计学验证实验。结果表明,最小区分量为9.71%,8通道结构的检测通量约为370个/min。高通量检测及表征方法可以实现不同变形性红细胞的统计学有效区分,为其他类型样本的高通量变形性分析提供了解决思路。

A high throughput measurement method of human red blood cells(RBCs)deformability combined with optical tweezers technology and the microfluidic chip was proposed to accurately characterize the deformability of RBCs statistically.Firstly,the effective stretching deformation of RBCs was realized by the interaction of photo-trapping force and fluid viscous resistance.Secondly,the characteristic parameters before and after the deformation of the single cell were extracted through the image processing method to obtain the deformation index of area and circumference.Finally,statistical analysis was performed,and the average deformation index parameters(,)were used to characterize the deformability of RBCs.A high-throughput detection system was built,and the optimal experimental conditions were obtained through a large number of experiments.Three groups of samples with different deformability were used for statistical verification.The results showed that the smallest cell component was 9.71%,and the detection flux of 8-channel structure was about 370 cells/min.High-throughput detection and characterization methods can effectively distinguish different deformed RBCs statistically,which provides a solution for high-throughput deformation analysis of other types of samples.