TH17细胞平衡及免疫微环境在过敏性哮喘发病中的作用

TH17 cells balance and mechanism in the immune microenvironment on the pathogenesis of allergic asthma

目的探讨辅助性T细胞17(TH17)及其相关细胞因子表达水平在过敏性哮喘发病中的作用机制。方法选取120例哮喘患者作为本文的研究对象。根据患者的哮喘类型,将120例患者分为过敏性哮喘以及非过敏性哮喘两组,每组60例患者,同时选择同期入院体检的48例健康人群作为对照组。测定三组受试者外周血中Th 17、Treg、Th2比例以及血浆和诱导痰中IL-17A、IL-4、IL-5、IL-10及IL-18水平。此外测定三组患者诱导痰中中性粒细胞、嗜酸粒细胞比例。结果过敏性哮喘组Th17比例高于对照组(P<0.05);过敏性哮喘组Treg比例低于对照组(P<0.05);过敏性哮喘组Th17比例低于非过敏性哮喘组(P<0.05);三组间Th2比例无统计学意义(P>0.05)。过敏性哮喘组IL-17A、IL-4以及IL-5水平高于对照组(P<0.05);过敏性哮喘组IL-10及IL-18水平低于对照组(P<0.05),过敏性哮喘组IL-17A水平低于非过敏性哮喘组(P<0.05);过敏性哮喘组IL-4水平高于非过敏性哮喘组(P<0.05),过敏性哮喘组IL-17A、IL-4、IL-5、IL-10及IL-18水平均高于对照组。过敏性哮喘组IL-17A以及IL-18水平低于非过敏性哮喘组(P<0.05);过敏性哮喘组IL-4水平高于非过敏性哮喘组(P<0.05);两组间IL-5、IL-10水平无统计学意义(P>0.05)。过敏性哮喘组中性粒细胞、嗜酸粒细胞比例均高于对照组(P<0.05),过敏性哮喘组中性粒细胞比例低于非过敏性哮喘组,且差异存在统计学意义(P<0.05);过敏性哮喘组嗜酸粒细胞比例高于非过敏性哮喘组(P<0.05)。结论TH17及其相关细胞因子的免疫失衡与过敏性哮喘的发生密切相关。

Objective To investigate the T helper cells 17 and the related cytokines expression level in the role of allergic asthma pathogenesis mechanism.Methods This study selected 120 cases of asthma patients as the research object of this study.Depending on the type of asthma patients,120 patients were divided into allergic asthma and allergic asthma in the two groups,each group of 60 patients,hospital medical examination at the same time;choose the same period of 48 cases of healthy people as control group.Determination of Th17 and Treg in three groups of participants were peripheral blood,Th2 ratio and serum and induced sputum IL-17A,IL-4,IL-5,IL-10 and IL-18 levels.In addition,determination of neutrophil in induced sputum three groups of patients,acidophilic granulocyte ratio.Results Th17 ratio in allergic asthma group was higher than that in control group(P<0.05).Treg ratio in allergic asthma group was lower than that in control group(P<0.05).Th17 ratio in allergic asthma group was lower than that in non-allergic asthma group(P<0.05).There was no significant difference in Th2 ratio among the three groups(P>0.05).The levels of IL-17A,IL-4 and IL-5 in the allergic asthma group were higher than those in the control group(P<0.05).The levels of IL-10 and IL-18 in the allergic asthma group were lower than those in the control group(P<0.05).The levels of IL-17A in the allergic asthma group were lower than those in the non-allergic asthma group(P<0.05);the levels of IL-4 in the allergic asthma group were higher than those in the non-allergic asthma group.The levels of IL-17A,IL-4,IL-5,IL-10 and IL-18 in asthma group were higher than those in control group.The levels of IL-17A and IL-18 in allergic asthma group were lower than those in non-allergic asthma group(P<0.05).The levels of IL-4 in allergic asthma group were higher than those in non-allergic asthma group(P<0.05).There was no significant difference in the levels of IL-5 and IL-10 between the two groups(P>0.05).The proportion of neutrophils and eosinophils in allergic asthma group was higher than that in control group(P<0.05).The proportion of neutrophils in allergic asthma group was lower than that in non-allergic asthma group(P<0.05).The proportion of eosinophils in allergic asthma group was higher than that in non-allergic asthma group(P<0.05).Conclusion TH17 and other cytokine immune imbalance is closely related to the occurrence of allergic asthma.