骨内微血管显影及三维重建在激素致大鼠股骨头缺血性坏死模型中的应用

Application of microvascular imaging and three-dimensional reconstruction in evaluation of steroid induced avascular necrosis of femoral head in rats

目的了解Micro-CT在大鼠激素性股骨头缺血性坏死模型中的应用价值。方法 20只SD大鼠随机分为实验组和正常组,实验组腹腔注射醋酸泼尼松龙进行股骨头缺血性坏死造模。两组分别于2、4、6、8周进行Micro-CT扫描。结果正常组与实验组间各项骨组织测量指标比较差异均有统计学意义(P<0.01或0.05)。2周出现骨小梁稀疏、断裂,关节面边缘模糊,软骨下囊性改变,部分血管萎缩、变细;第4周骨小梁进一步减少,部分皮质骨缺损形成小空洞,大部分血管萎缩,微血管部分中断;第6周骨小梁数目明显减少,皮质骨下空洞扩大,血管萎缩加重,大部分微血管中断;第8周骨小梁中断稀薄,关节面下较大骨缺损,微血管不显影。结论 Micro-CT可用于动态观察大鼠激素性股骨头缺血性坏死结构变化。

Objective To study the application of Micro-CT in steroid induced avascular necrosis of femoral head(ANFH)in rats.Methods Twenty SD rats were randomly divided into ANFH and control groups.ANFH model was established by intraperitoneal injection of prednisolone acetate.All rats underwent Micro-CT scanning in 2,4,6,and 8 weeks.Results Bone histomorphometric parameters were different between two groups at all timepoints(P<0.01 or 0.05).In two weeks,bone trabeculae were sparse and fractured,with fuzzy articular surface edges,subchondral cystic changes,and partial vascular atrophy.In four weeks,bone trabeculae were further reduced,with partial cortical defects and small cavities,extensive vascular atrophy,and partial microvascular interruption.In six weeks,bone trabeculae were remarkably decreased,with enlarged subcortical cavities,severe vascular atrophy,and extensive microvascular interruption.In eight weeks,bone trabeculae were interrupted and scarce,with large bone defects below articular surface and absent microvessels.Conclusion The Micro-CT is feasible to observe the dynamical changes of steroid induced ANFH in rats.