SPHK1与MMP2蛋白在结肠癌组织中的表达及临床意义

Expression and clinical significance of SPHK1 and MMP2 in human colorectal carcinoma tissues.

目的探讨鞘氨醇激酶1(SPHK1)与基质金属蛋白酶2(MMP2)在人结肠癌组织中的表达及其与临床病理特征之间的关系。方法2014年1月至2018年12月于宝鸡市中心医院行结肠癌根治术患者共201例,使用免疫组织化学法检测SPHK1蛋白与MMP2蛋白在结肠癌组织中的表达情况,使用χ2检验分析其与临床各病理资料之间的关系。结果SPHK1蛋白在结肠癌组织中的表达率为54.7%,明显高于癌旁组织中的39.7%,差异具有统计学意义(P<0.05);SPHK1蛋白的高表达与患者的淋巴结是否转移、T分期、组织学分化程度以及是否血管侵犯有关(P<0.05);MMP2在结肠癌组织中的表达率为64.7%,明显高于癌旁组织中的24.4%,差异具有统计学意义(P<0.05);MMP2蛋白的高表达与淋巴结转移与否、T分期、M分期以及血管侵犯有关(P<0.05);SPHK1蛋白在结肠癌组织中的表达与MMP2蛋白呈正相关(r=0.352,P<0.05)。结论SPHK1蛋白与MMP2蛋白在人结肠癌组织中均异常表达,且可能参与到结肠癌的侵袭与转移过程中。

Objective To investigate the expression of Sphingosine kinase 1(SPHK1)and matrix metalloproteases 2(MMP2)in human colorectal carcinoma tissues and analyze its association with clinic pathologic features.Methods There were 201 patients who diagnosed for colon cancer collected in Baoji Central Hospital from January 2014 to December 2018.Immunohistochemistry was carried out to examine the expression of SPHK1 and MMP2 in colorectal carcinoma tissues,the relationship between them and clinical pathological data was analyzed byχ2 test.Results The expression rate of SPHK1 in colorectal carcinoma tissues was 54.7%,which was significantly higher than 39.7%in non-tumor tissues(P<0.05).The expression of SPHK1 was closely correlated with T stage,N stage,histologic differentiation and vascular invasion(P<0.05).The expression of MMP2 in colorectal carcinoma tissues was 64.7%versus 24.4%in the adjacent tissues(P<0.05).The expression of MMP2 was closely correlated with T stage,M stage,and vascular invasion(P<0.05)The expression of SPHK1 was positive correlated with MMP2 expression in colorectal carcinoma tissues(r=0.352,P<0.05).Conclusion SPHK1 and MMP2 protein show abnormal expression in human colorectal carcinoma tissues and may be involved in the invasion and metastasis of colon cancer.