青少年期及青春期后寻常痤疮患者肠道菌群及宿主-肠道共代谢产物差异分析

Altered Gut Microbiota and Microbial Metabolites Observedin Pre/peri-pubertal and Postpubertal Adolescence(or in Early and Late Preadolescence)of Patients with Acne Vulgaris

目的本研究旨在研究青少年期及青春期后寻常痤疮患者肠道菌群和肠道-宿主共同代谢产物的特点。方法回顾性分析43例未经治疗的寻常痤疮患者与43例年龄、性别匹配的健康对照组,分别收集他们的粪便标本,提取细菌DNA,基于16S rDNA测序分析肠道菌群;采用气相色谱仪对118种肠道-宿主共代谢产物进行定量分析。结果α多样性分析结果显示:与健康对照组相比,无论青少年期还是青春期后寻常痤疮患者的肠道菌群多样性均降低。与健康对照组相比,青少年期痤疮患者肠道中拟杆菌门(Bacteroidetes)、疣微菌门(Verrucomicrobia)增多,厚壁菌门(Firmibutes)和念珠菌(Candidatus Saccharibacteria)水平明显降低。在属的水平,青少年患者比健康对照有18种细菌水平明显降低。但是,在青春期后痤疮患者和健康对照之间没有发现差异细菌。肠道-宿主共代谢产物分析发现:青少年痤疮患者和健康组之间发现了8种差异代谢物,在青春期后患者和健康组之间发现了6种差异代谢物。结论相对于青春期后寻常痤疮患者,青少年期患者肠道菌群及肠道代谢产物失调更加显著。

Objective This study was conducted to identify characteristics of gut microbiota and microbial metabolites in peri-pubertal and post-pubertal adolescence of patients with acne vulgaris.Methods Gut microbiota were analyzed by sequencing 16S DNA genes in fecal samples of 43 acne vulgaris patients and 43 healthy controls.Targeted quantitative analyses of 118 gut microbial metabolites were performed using gas chromatography time-of-flight mass spectrometry.Resultsα-diversity of the gut microbime showed less diversity of gut microbiota in peri-pubertal and post-pubertal patients than in matched healthy controls.We identified increased Bacteroidetes,Verrucomicrobia,decreased Firmibutes,and Candidatus Saccharibacteria at phyla level and decreased 18 microbes at genus level in peripubertal acne patients group compared to matched healthy controls.However,no significant differences were found between post-puberal acne patients and the matched healthy controls.Targeted analysis of gut microbial metabolites demonstrated eight different metabolites between the peripubertal acne patients and healthy controls while six different ones between the post-pubertal acne cases and healthy controls.Conclusion Peripubertal acne patients had a significantly altered gut microbiome and metabolites compared to healthy controls and post-pubertal acne patients,suggesting that the gut microbial dysbiosis may contribute to the pathogenesis of peripubertal acne more than that of pos-pubertal acne.