隐丹参酮增强胆管癌吉西他滨化疗敏感性

Cryptotanshinone enhances the chemosensitivity of gemcitabine in cholangiocarcinoma

目的:探讨隐丹参酮增强胆管癌QBC939细胞对吉西他滨化疗敏感性的作用及其机制。方法:以人胆管癌QBC939细胞作为研究对象,将实验分为4组:对照组、隐丹参酮组、吉西他滨组和联合用药组。用CCK-8法检测细胞生长抑制率,Annexin V-FITC/PI双标记流式细胞术检测细胞凋亡,Western blot法检测凋亡相关蛋白表达水平。结果:经24 h药物处理后,隐丹参酮终浓度为10、30、50μmol/L时,细胞生长抑制率分别为(22.30±2.15)%、(42.53±3.62)%、(51.85±4.81)%,低于对照组的(99.22±3.52)%,差异均有统计学意义(P<0.05);经24 h药物处理后,联合用药组的细胞凋亡率为(35.40±1.57)%,高于隐丹参酮组的(19.80±0.92)%、吉西他滨组的(24.20±0.56)%和对照组的(8.84±0.58)%,差异均有统计学意义(P<0.05);Western blot结果显示联合用药组中的Active Caspase-3及Bax蛋白表达高于吉西他滨组、隐丹参酮组及对照组,差异均有统计学意义(P<0.05)。结论:隐丹参酮可增加吉西他滨在胆管癌细胞的化疗敏感性。

Objective:To investigate the effect and mechanism of cryptotanshinone on the chemosensitivity of QBC939 cells to gemcitabine.Methods:The human cholangiocarcinoma QBC939 cells were divided into four groups:control group,cryptotanshinone group,gemcitabine group and cryptotanshinone+gemcitabine group.CCK-8 method was used to detect the inhibition rate of cell growth.Apoptosis was detected by flow cytometry.Western blot was used to detect the expression of apoptosis related protein.Results:After 24 h treatment,when the final concentration of cryptotanshinone was 10,30 and 50μmol/L,the inhibition rate of cell growth was 22.30%±2.15%,42.53%±3.62%and 51.85%±4.81%respectively,which was lower than that of the control group(99.22%±3.52%),with statistical difference(P<0.05).After 24 h treatment,the apoptosis rate of cryptotanshinone+gemcitabine combination group was 35.40%±1.57%,which was higher than the cryptotanshinone group(19.80%±0.92%),the gemcitabine group(24.20%±0.56%)and the control group(8.84%±0.58%).The difference was statistically significant(P<0.05).Western blot showed that the expression of active caspase-3 and Bax protein in the combination of cryptotanshinone and gemcitabine group was higher than that in gemcitabine group,cryptotanshinone group and control group.Conclusion:Cryptotanshinone can increase the chemosensitivity of gemcitabine in cholangiocarcinoma cells.