功能光学成像——从单分子到人眼

Functional Optical Imaging——From Single Molecules to Human Eyes

超分辨定位成像打破了衍射极限,使得光学成像系统的分辨率可以达到20-50 nm。传统的超分辨定位成像无法获取荧光分子的光谱信息,因此,研发了一种基于光栅的光谱光子定位超分辨成像技术(SPLM),能够同时获取单个分子的空间位置信息和光谱信息。SPLM可以用于多分子超分辨成像,并且能够分辨发射光谱高度重叠的荧光标记物。此外,针对常用的光学诊断技术OCT,研发了一种可见光OCT(Vis-OCT),弥补了传统OCT无法用于氧代谢成像的缺陷,并将该技术用于致盲性疾病的研究。

Super-resolution localization imaging breaks the diffraction limit and enables optical imaging with a spatial resolution of 20 to 50 nanometers. However,traditional super-resolution imaging cannot analyse the spectral information of the fluorescent molecules. We developed spectroscopic photon localization microscopy(SPLM),which can simultaneously analyse the spatial and spectral information of single fluorescent molecule. Using SPLM,we demonstrated simultaneous multi-molecular super-resolution imaging,where the number of fluorescence labels can have largely overlapping emission spectra with only minute differences. Besides,for the optical coherent tomography(OCT),a commonly used optical diagnosis technology,we developed visible-light OCT or vis-OCT to remedy the limitation that the traditional OCT cannot used for oxygen metabolic imaging and we are applying vis-OCT to investigate blinding diseases.