弓形虫微线蛋白25的生物信息学分析及其互作蛋白预测

Bioinformatics analysis and prediction interaction protein of Toxoplasma gondii microneme protein 25

目的对弓形虫微线蛋白25(TgMIC25)进行生物信息学分析,并预测其互作蛋白。方法利用ExPASY在线软件对TgMIC25蛋白的理化性质、信号肽、跨膜结构域、结构域、二/三级蛋白结构、蛋白磷酸化位点、互作蛋白进行预测分析;利用SYFPEITHI和IEDB预测其B/T细胞抗原表位,分析其免疫原性。结果TgMIC25共编码302个氨基酸,相对分子质量为33.194×10^3,理论等电点为4.40。该蛋白无信号肽序列,在242-264 aa处存在一个潜在的跨膜区,主要是由EGF样结构域(Epidermal Growth Factor-like domain)组成,包含48个磷酸化位点,与TgMIC13为互作蛋白,具有多个B、T细胞抗原表位。结论生物信息学方法预测TgMIC25蛋白含有GEF样结构及个B、T细胞抗原表位,并与TgMIC13为互作蛋白,为该蛋白后续试验研究奠定基础。

Objective To bioinformatically analyze and predict the interaction proteins of Toxoplasma gondii microneme protein 25.Methods T.gondii microneme protein 25 was subjected to predictive analysis of its physicochemical properties,signal peptides,transmembrane domains,motifs,secondary and tertiary structures,protein phosphorylation sites,and interaction proteins using the online software ExPASY.The B and T cell epitopes of TgMIC25 were predicted using SYFPEITHI and IEDB in order to analyze its immunogenicity.Results TgMIC25 encodes a total of 302 amino acids,and it has a molecular weight(Mr)of 33.194×10^3 and a theoretical isoelectric point of 4.40.The protein has no signal peptide sequences and a potential transmembrane region at amino acids 242-264.TgMIC25 mainly contained an epidermal growth factor-like domain and it had 48 phosphorylation sites.A possible interaction protein is TgMIC13 in T.gondii.TgMIC25 contained multiple B and T cell epitopes.Conclusion Systematic bioinformatic analysis of TgMIC25 in this study has laid the foundation for subsequent experimental study.