CAⅨ、E-cadherin和β-catenin在胃癌组织中的表达及意义

The expression and clinical significance of CAⅨ,E-cadherin and β-catenin in gastric cancer

目的探讨碳酸酐酶Ⅸ(CAⅨ)、E-钙黏蛋白(E-cadherin)和β-连环蛋白(β-catenin)对胃癌的诊断及预后的判断价值。方法选取2008年1月至2011年12月如皋市人民医院普外科行胃癌根治术患者80例为研究组(取其手术切除的胃腺癌病理标本),及同期50例胃镜检查正常者为对照组(取其黏膜活检正常的病理标本)为研究对象,采用免疫组化SP法检测两种组织中CAⅨ、E-cadherin和β-catenin的表达情况,分析它们与胃癌临床病理特征及预后之间的关系。随访终点为患者死亡或术后60个月。结果在研究组胃癌组织中,CAⅨ的阳性表达率及E-cadherin、β-catenin的异常表达率(染色强度弱、阳性细胞数低和异位表达)均显著高于对照组正常胃黏膜组织(P均<0.01)。CAⅨ在胃癌组织中的阳性表达与肿瘤直径、肌层浸润深度、淋巴结转移及肿瘤临床分期相关(P<0.05,P<0.01);E-cadherin和β-catenin在胃癌组织中的异常表达与肌层浸润深度、病理分化程度、淋巴结转移及肿瘤临床分期相关(P<0.05,P<0.01)。胃癌组织中CAⅨ阳性表达与E-cadherin、β-catenin异常表达分别呈正相关(r=0.223、0.610,P均<0.05),胃癌组织中E-cadherin异常表达与β-catenin异常表达呈正相关(r=0.434,P<0.05)。CAⅨ阳性表达的胃癌患者生存率明显低于阴性表达者(P<0.05),E-cadherin及β-catenin异常表达的胃癌患者生存率明显低于正常表达者(P均<0.05)。结论胃癌组织中CAⅨ阳性高表达,E-cadherin及β-catenin异常表达。三者的表达情况与胃癌患者生存密切相关。监测CAⅨ、E-cadherin和β-catenin对胃癌的诊断及预后判断具有一定的价值。

Objective To investigate the value of carbonic anhydraseⅨ(CAⅨ),E-cadherin and β-catenin in the diagnosis and prognostic prediction of gastric cancer.Methods From January 2008 to December 2011,80 patients with gastric adenocarcinoma surgically removed pathological specimens and 50 pathological specimens with normal mucosal biopsy under gastroscopy were selected as the research objects. and 50 cases of pathological specimens with normal gastroscopic mucosal biopsy under gastroscopy during the same period were selected as the research objects.The expression of CAⅨ、E-cadherin and β-catenin were detected by immunohistochemical SP in two kinds of tissues to analyze their relationship with clinicopathological features and prognosis.Results The positive expression rate of CAⅨ and abnormal expression rate of E-cadherin and β-catenin in gastric cancer tissues were significantly higher than those in normal gastric mucosa tissues(all P<0.01).The positive expression of CAⅨ in gastric cancer tissues was related to tumor diameter,depth of muscle layer invasion,lymph node metastasis and clinical stage of the tumor(P<0.05,P<0.01);the abnormal expression of E-cadherin and β-catenin were related to tumor invasion depth,histological grade,lymph node metastasis and clinical stage of the tumor(P<0.05,P<0.01).In gastric cancer,the positive expression of CAⅨ was positively correlated with the abnormal expression of E-cadherin and β-catenin(r=0.223, P<0.05;r=0.610,P<0.05)respectively, while there was a positive correlation between abnormal expression of E-cadherin and β-catenin(r=0.434,P<0.05).The 5-year survival rate of gastric cancer patients with CAⅨ positive expression was lower than that with negative expression(P<0.05);the survival rate of expression gastric cancer patients with abnormal of E-cadherin and β-catenin were lower than that with normal expression(all P<0.05).Conclusions The high expression of CAⅨ and the abnormal expression of E-cadherin and β-catenin exist in gastric cancer tissues.The expression of the three is closely related to the survival of gastric cancer patients.Monitoring CAⅨ,E-cadherin and β-catenin has a certain value in the diagnosis and prognostic prediction of gastric cancer.