宫颈癌组织中AEG-1和TLR3表达与其微血管密度及预后的相关性

Associations of AEG-1 and TLR3 expressions with the intratumoral microvessel density and prognosis in patients with cervical cancer

目的探讨宫颈癌组织中星形细胞上调基因(AEG)-1、Toll样受体(TLRs)3表达与其微血管密度(MVD)及预后的相关性。方法选取2011年12月至2014年12月收治的112例宫颈癌患者为观察组(取其宫颈癌病灶标本),另选子宫肌瘤切除术患者74例为对照组(取其正常宫颈组织标本),对比两种组织AEG-1、TLR3表达水平及MVD,Pearson分析AEG-1、TLR3表达与MVD的相关性;分析宫颈癌预后影响因素,对比不同预后患者AEG-1、TLR3表达水平;Spearman分析宫颈癌组织中AEG-1、TLR3表达与预后影响因素的相关性;以宫颈癌组织中AEG-1、TLR3表达量均数划分为高表达者与低表达者,用直接计算法计算并对比不同AEG-1、TLR3表达者5年生存率。结果观察组AEG-1表达水平和MVD高于对照组,TLR3表达水平低于对照组(P均<0.01)。宫颈癌组织中AEG-1表达与MVD呈正相关关系(r=0.607,P<0.05),TLR3表达与MVD呈负相关关系(r=-0.466,P<0.05)。病理分期、淋巴结转移、分化程度、肿瘤直径是宫颈癌预后重要影响因素(P<0.05,P<0.01);AEG-1与病理分期、淋巴结转移、肿瘤直径呈正相关,与分化程度呈负相关(P均<0.01);TLR3与病理分期、淋巴结转移、肿瘤直径呈负相关,与分化程度呈正相关(P均<0.01)。AEG-1高表达者5年生存率低于低表达者(57.97%vs93.02%,χ^2=15.949,P<0.01);TLR3高表达者5年生存率高于低表达者(95.12%vs57.75%,χ^2=17.791,P<0.01)。结论宫颈癌组织中AEG-1表达与肿瘤MVD呈正相关,TLR3表达与MVD呈负相关,二者均参与肿瘤侵袭、转移过程,可作为评价宫颈癌预后的重要指标。

Objective To investigate the associations of the expressions of astrocyte upregulated genes(AEG)-1 and Tolllike receptors(TLRs)-3 in cervical cancer tissue with intratumoral microvascular density(MVD)and prognosis in patients with cervical cancer.Methods A total of 112 cervical cancer patients treated from December 2011 to December 2014 were selected as the observation group(the specimens of cervical cancer tissue were taken),and 74 patients with hystero myomectomy were selected as the control group(the normal cervical tissue samples were taken).After detecting and comparing the expressions of AEG-1 and TLR3 and MVD in two groups,Spearman was used to analyze the associations of the expression of AEG-1 and TLR3 with MVD and with the prognostic factors of cervical cancer.According to the mean expressions of AEG-1 and TLR3 in cervical cancer,the patients were divided into high-expression group and low-expression group.The 5-year survival rates of patients with high and low expression of AEG-1 and TLR3 were calculated and compared by direct calculation method.Results Compared with control group,AEG-1 expression level and MVD increased,while TLR3 expression level decreased in observation group(all P<0.01).There was a positive correlation between AEG-1 expression and MVD(r=0.607,P<0.05)and a negative correlation between TLR3 expression and MVD(r=-0.466,P<0.05).Pathological stage,lymph node metastasis,differentiation degree and tumor diameter were the important prognostic factors of cervical cancer(P<0.05,P<0.01).AEG-1 was positively correlated with pathological stage,lymph node metastasis and tumor diameter and negatively correlated with differentiation degree of tumor(all P<0.01);TLR3 was negatively correlated with pathological stage,lymph node metastasis and tumor diameter and positively correlated with differentiation degree of tumor(all P<0.01).The 5-year survival rate of patients with high expression of AEG-1 was lower than that of patients with low expression of AEG-1(57.97%vs 93.02,χ^2=15.949,P<0.01);the 5-year survival rate of TLR3 high-expression group was higher than that of low-expression group(95.12%vs 57.75%,χ^2=17.791,P<0.01).Conclusions The expression of AEG-1 is positively correlated with MVD,and the expression of TLR3 is negatively correlated with MVD in cervical cancer tissues.Both AEG-1 and TLR3 are involved in the process of tumor invasion and metastasis and can be used as the important indicators to evaluate the prognosis of cervical cancer.