高效液相色谱法测定瑶药虎刺中熊果酸的含量

Determination of ursolic acid in Damnacanthus indicus of Yao medicine by HPLC

目的建立高效液相色谱法(HPLC)测定瑶药虎刺中熊果酸的含量。方法采用HPLC法测定瑶药虎刺中熊果酸的含量。色谱柱为Agilent Zorbax SB-C18(250 mm×4.6 mm,5μm),流动相为甲醇∶乙腈∶0.2%磷酸溶液(18∶67∶15),流速1.0 mL·min^-1,柱温25℃,检测波长为210 nm。结果熊果酸在11.13~111.3μg·mL-1范围内线性关系良好(r=0.9998),平均回收率为98.67%(RSD=2.72%)。结论采用HPLC法测定瑶药虎刺中熊果酸的含量操作简单,重现性好,可为瑶药虎刺的质量控制和开发利用提供科学依据。

Objective To establish a HPLC method for determining ursolic acid in Yao medicine of Damnacanthus indicus.Methods The chromatographic column was Agilent Zorbax SB-C18 column(250 mm×4.6 mm,5μm),and the mobile phase was consisted of methanol∶acetonitrile∶0.2%phosphoric acid(18∶67∶15).The flow rate was 1.0 mL·min^-1.The column temperature was set at 30℃,and the detection wavelengths were 210 nm.Results A good linearity of ursolic acid was in the range of 11.13~111.3μg·mL-1(r=0.9998)and the average recovery was 98.67%(RSD=2.72%).Conclusion The HPLC method is simple and reproducible,which can provide scientifical basis for the quality control and development of Damnacanthus indicus in Yao medicine.