南瓜干旱胁迫相关基因的转录组分析

Transcriptome Analysis of Pumpkin Under Drought Stress

南瓜是一种重要的经济作物,干旱是南瓜生产的主要限制因素之一。为探索南瓜耐旱的分子机制和挖掘耐旱相关基因,本研究通过PEG-6000模拟干旱胁迫处理南瓜品种金贝1号,对不同时期南瓜叶片进行高通量测序。结果表明:共获得1254个共表达差异基因,通过基因功能注释和富集分析,126个基因参与耐旱生物进程。值得关注的是,其中表达量最高的5个基因均参与糖酵解通路,因此,推测糖酵解是南瓜的耐旱分子机制的重要途径。

Pumpkin is one of the important economic crops in northeast China,drought is the main limiting factor for pumpkin production.In order to explore the molecular mechanism of drought tolerance and drought tolerance related genes in pumpkin,pumpkin variety Jinbei No.1 was treated with PEG-6000 to simulate drought stress,high throughput sequencing of pumpkin leaves at different stages was carried out.The results showed that 1254 differentially expressed genes were obtained,and 126 genes were involved in the biological process of drought tolerance through gene function annotation and enrichment analysis.It is worth noting that the five genes with the highest expression levels were involved in the glycolysis pathway.Therefore,it is speculated that glycolysis is an important pathway in the molecular mechanism of drought tolerance in pumpkin.