FHIT及Apollon蛋白在结直肠癌组织中的表达特征及预后预测价值

Expression and prognostic value of FHIT and Apollon proteins in colorectal cancer tissue

目的:探讨脆性组氨酸三联体(FHIT)及凋亡抑制蛋白Apollon在结直肠癌(CRC)组织中的表达特征及预后预测价值。方法:回顾性分析163例CRC患者癌组织、癌旁组织中FHIT及Apollon蛋白的表达情况;分析两蛋白表达与病理参数的关系及其对预后的影响。结果:CRC组织中,FHIT和Apollon蛋白阳性率分别为32.5%和84.7%,癌旁组织中分别为67.5%和48.5%;与癌旁组织比较,CRC组织中FHIT蛋白阳性率降低,Apollon蛋白阳性率升高(P<0.001);癌组织中FHIT与Apollon蛋白表达存在负关联(rP=-0.183,P=0.017)。与浸润至肌层或浆膜外、Dukes分期A或B期、无淋巴结转移、无SCRLM的CRC组织比较,浸润至浆膜层、Dukes分期C或D期、有淋巴结转移、有SCRLM的CRC组织中FHIT阳性率降低,Apollon阳性率升高(P<0.05)。FHIT+Apollon-患者预后优于FHIT+Apollon+患者,FHIT-Apollon+、FHIT-Apollon-患者预后差于FHIT+Apollon+患者(P<0.001),FHIT-Apollon+与FHIT-Apollon-患者预后比较差异无统计学意义(P>0.05)。结论:FHIT、Apollon蛋白表达与CRC侵袭、转移及预后关系密切,两者可作为预测CRC转移及预后的可靠标志物。

Aim:To investigate the expression characteristics and prognostic value of fragile histidine triad(FHIT)and apoptosis inhibitory protein Apollon in colorectal cancer(CRC).Methods:The expressions of FHIT and Apollon proteins in CRC and paracancerous tissue from 163 patients with CRC were detected.The relationship of their expressions in CRC tissue and pathological parameters,and their effects on prognosis were analyzed.Results:The positive expression rates of FHIT and Apollon proteins were 32.5%and 84.7%in CRC tissue,67.5%and 48.5%in the paracancerous tissue.Compared with the paracancerous tissue,the positive rate of FHIT was lower,while that of Apollon protein was higher(P<0.001)in CRC tissue;the expressions of the two proteins in CRC tissue were negatively associated(rP=-0.183,P=0.017).Compared with patients with infiltration to the muscular layer or outer serosa,Dukes stage A or B,no lymph node metastasis,and no SCRLM,the FHIT positive rates were lower and the Apollon positive rates were higher in the patients with infiltration to serosa,Dukes stage C or D,with lymph node metastasis and SCRLM(P<0.05).FHIT+Apollon-patients had better prognosis than FHIT+Apollon+patients(P<0.001).FHIT-Apollon+,FHIT-Apollon-patients′prognosis was worse than FHIT+Apollon+patients(P<0.001).There was no significant difference in prognosis between FHIT-Apollon+and FHIT-Apollon-patients(P>0.05).Conclusion:FHIT and Apollon protein expression is abnormal,and it is closely related to CRC invasion,metastasis and prognosis,which can be used as reliable markers for predicting CRC metastasis and prognosis.