汤姆青霉的抗菌活性研究

A Study on Antibacterial Activity of Penicillium thomii

本试验采用滤纸片法和牛津杯法测定了汤姆青霉PT95(Penicillium thomii PT95)发酵液对金黄色葡萄球菌(Staphyloccocus aureus)、枯草芽孢杆菌(Bacillus subtilis)、大肠杆菌(Escherichia coli)、酿酒酵母(Saccharomyces cerevisiae)、黑根霉(Rhizopus nigricans)、德氏乳杆菌(Lactobacillus delbrueckii)、植物乳杆菌(Lactobacillus plantarum)的抑制作用,从而筛选出抑菌效果最佳的菌株。同时测定了汤姆青霉PT95发酵液的最小抑菌质量浓度,并以抑菌圈直径大小为指标,采用单因素试验对碳源、氮源、无机盐等培养条件进行优化。结果表明:汤姆青霉PT95对枯草芽孢杆菌的抑制作用最为明显,抑菌率最大且高达65.4%。最优培养基配方为葡萄糖8.64 g/L、蛋白胨5.00 g/L、MgSO4·7H2O 0.30 g/L、NaCl 3.00 g/L、琼脂20.00 g/L、pH7.0~7.2。汤姆青霉PT95菌株的发酵产物中存在抑菌物质,通过优化培养有助于提高汤姆青霉PT95菌株的抑菌活性。

The effects of Penicillium tomii PT95 strain on Staphylococcus aureus,Bacillus subtilisus,Escherichia coli,Saccharomyces cerevisiaeae,Rhizopus nigricansa,Lactobacillus delbrueckii,Lactobacillus plantarum were studied by filter paper method and oxford cup method,the methods can screen out the most effective strain.At the same time,the minimum concentration of fermentation filtrate of Penicillium thomii was determined.Taking the diameter of bacteriostatic ring as an index,single factor test was used to optimize the culture conditions of carbon source,nitrogen source and inorganic salt etc.The results showed that Penicillium thomii PT95 strain had the most inhibitory effect on Bacillus subtilis,with the largest inhibition rate and up to 65.4%.The optimized medium was glucose 8.64 g/L,peptone 5.00 g/L,MgSO4·7H2O 0.30 g/L,NaCl 3.00 g/L,agar 20.00 g/L,pH7.0~7.2.Inhibitory substance was found abundantly in secondary metabolites of Penicillium thomii PT95 strain.Antibacterial activity was improved by optimal production conditions.