HA/ZnO/SiO2梯度涂层材料的制备及其体外抗菌活性测试

Preparation and Bioactivity Test in Vitro of HA/ZnO/SiO2 Gradient Coating Materials

采用区带电泳法电泳羟基磷灰石(HA)、壳聚糖(CS)、ZnO和SiO2悬浊液,接着施予反向电场进行电泳沉积从而在钛基材表面获得HA/CS/ZnO/SiO2复合涂层材料,而后经710℃焙烧2 h后得到HA/ZnO/SiO2涂层材料(Z1)。同时也仅用电泳沉积法直接制得HA/ZnO/SiO2涂层材料(E1)作为对比。X射线能谱分析发现Si和Zn两种元素在Z1的径向上均呈现梯度分布,初步说明Z1具有一定的梯度。结合扫描电子显微镜、粉末X射线衍射、傅里叶红外光谱等手段对2种材料进行表征,发现Z1的各种性能较E1更为优异,主要表现在Z1与钛基材表面的结合强度达31.2 MPa,且在HEPES模拟体液中培养14 d后Z1表面碳磷灰石化程度更加完全。另外,抑菌实验发现Z1粉末对大肠杆菌和金黄色葡萄球菌的抑菌率分别为81.7%和89.4%。

Hydroxyapatite(HA),chitosan(CS),ZnO and SiO2 suspensions were distributed by zonal electrophoresis,and then HA/CS/ZnO/SiO2 composite coating material on the Ti⁃based surface was obtained by electrophoretic depo⁃sition in a reverse electric field subsequently.An HA/ZnO/SiO2 coating material(Z1)was fabricated by calcination at 710℃for 2 h.At the same time,another HA/ZnO/SiO2 coating material(E1)was directly prepared only by elec⁃trophoretic deposition for comparison.Both Si and Zn elements with a gradient distribution in the radial direction of the coating was found by X⁃ray energy spectrum analysis,which indicates that Z1 has certain gradient properties.Then,scanning electron microscope,powder X⁃ray diffraction and Fourier transform infrared spectroscopy were used to characterize the two coating materials.It was found that Z1 has more excellent properties than E1,mainly manifested in the bonding strength of Z1 and Ti substrate up to 31.2 MPa,and the degree of carbonated apatite on the surface of Z1 was more complete after 14 days of culture in HEPES simulated body fluid.In addition,the bacte⁃riostatic effect test of Z1 showed that the bacteriostatic rates of Z1 powder against E.coli and S.aureus were up to 81.7%and 89.4%,respectively.