辣木籽水提物对酒精诱导线虫运动功能障碍的影响

Moringa oleifera seed water extract protects alcohol-induced movement disorder in Caenorhabditis elegans

用不同浓度的辣木籽水提物干预醉酒秀丽隐杆线虫,观察其运动情况,并探究其组织内部乙醇浓度、钙调神经磷酸酶及乙酰胆碱酯酶活的变化情况,以探究辣木籽水提物对醉酒线虫生理变化的影响。结果显示,辣木籽水提物干预浓度为10mg/mL时,线虫的运动轨迹、摆头频率及吞咽频率与对照组线虫相比,其运动能力显著提高,但与正常组线虫无明显差异。辣木籽水提物能抑制乙醇在线虫体内的积累及钙调神经磷酸酶的过表达,增强乙酰胆碱酯酶的酶活力,以提高线虫抗酒精胁迫能力。说明低浓度的辣木籽水提物有缓解醉酒线虫运动障碍的作用。

It was investigated the alleviating effect of water-extracts from Moringa oleifera seeds(MWE)on alcohol damage,and explored the effects of Moringa seeds on the physiological changes of drunk nematodes.Different concentrations of MWE were used to interfere with nematodes drunkenness.Its movement,tissue ethanol concentration,calcineurin and acetylcholinesterase activity were evaluated.The results showed that the intervention concentration of MWE was 10 mg/mL,and the movement trajectory,frequency of head swinging and swallowing frequency of the nematode were significantly improved,compared with the control group.However,no significant difference was found compared to the un-treated group.MWE reduced the tissue ethanol concentration and the overexpression of calcineurin,increased the activity of acetylcholinesterase,and the resistance against alcohol stress in nematodes.These results suggest that the low concentration of MWE can protect alcohol-induced movement disorder in Caenorhabditis elegans.