菊芋多聚果糖的纯化技术研究

Purification of polyfructose from Jerusalem artichoke

以菊芋多聚果糖粗品为原料,采用模拟移动色谱技术纯化菊芋多聚果糖。在单柱色谱分离设备研究的基础上,研究旋转阀式模拟移动色谱技术与顺序式模拟移动床技术纯化菊芋多聚果糖的工艺参数。结果表明:顺序式模拟移动床技术是纯化芋多聚果糖的最优技术,最佳工艺参数为多聚果糖浓度30%,水料比(V水∶m料)1.1∶1.0(mL/g),在此条件下菊芋多聚果糖组分浓度为17.9%,含量达到96.9%,收率达到95.8%,较原料提高17.7%。

In this study,the Jerusalem artichoke polyfructose was purified by the simulated mobile chromatography(SMC)and sequential simulated mobile bed(SSMB)according to the results of single column chromatographic system,which the parameters of the SMB and SSMB were investigated.Based on the results,the SSMB was the better technology and the optimized process parameters were as follows,feed concentration of 30%,ratio of material to solvent of 1.1∶1.0.Under these conditions,the concentration of the Jerusalem artichoke polyfructose was 17.9%,the purity was 96.9%,and the recovery was 95.8%,which was 17.7%higher than the material.