大鼠颅脑损伤后差异表达基因及miRNA研究

Study on differentially expressed gene and miRNA after brain injury in rats

研究背景创伤性脑损伤(Traumatic brain injury, TBI)是致死率和致残率极高的外科疾患,我国在对于TBI的判断、治疗等方面还处于薄弱阶段,因此我们需要在分子层面了解大鼠颅脑损伤后基因及miRNA表达差异,以便更好地对症治疗。目的了解大鼠颅脑损伤后基因及miRNA表达差异,为临床治疗TBI提供新的思路。方法利用GEO2R筛选基因,然后用MiRwalk软件对筛选的miRNA的靶基因进行预测,再用DAVID做基因本体论功能富集分析,最后利用cytoscape做网络关系图。结果发现247个相对明显的差异表达的基因,包括150个上调表达基因和97个下调表达基因;7个差异表达的miRNA,包括2个上调表达miRNA和5个下调表达miRNA。这些差异表达基因在细胞内和细胞外都起作用,并且在炎症反应,药物应答等生物过程中起作用。将差异基因与靶基因对比后,可得到48个重合基因,同时发现这些重合基因与差异表达的miRNA有着一定的联系。

Traumatic brain injury(TBI) is a surgical disease with high mortality and disability rates. China is still in a weak stage in the judgment and treatment of TBI. It is necessary to understand the differentially expressed gene and miRNA after TBI at the molecular level,so as to provide basis for better treatment. This paper aims to investigate the differentially expressed gene and miRNA after TBI in rats, and provide a new way for clinical treatment of TBI. GEO2 R was used to screen genes,and mirwalk software was adopted to predict the target genes of the screened miRNA, Then, David was used to conduct gene ontology function enrichment analysis, and finally cytoscape was applied to carry out network analysis. Results show that 247 differentially expressed genes were found, including 150 up-regulated genes and 97 down-regulated genes, and 7 differentially expressed miRNAs were found, including 2 up-regulated miRNAs and 5 down-regulated miRNAs. These differentially expressed genes play a role in both intracellular and extracellular processes, as well as in biological processes such as inflammatory response and drug response. By comparing the difference gene and the target gene, 48 overlapped genes were obtained, and it was found that these overlapped genes had a certain relations with the differentially expressed miRNA.