西藏自治区马铃薯地方品种遗传多样性分析

Genetic Diversity Analysis of Local Potato Varieties in Tibet

为了探讨西藏自治区马铃薯地方品种的遗传多样性水平和遗传差异,利用基因测序分型(Genotyping-by-sequencing,GBS)技术对24个马铃薯地方品种和1个主栽品种进行遗传多样性分析。25个马铃薯品种观测杂合度(Ho)为0.24,期望杂合度(He)为0.19,有效等位基因数(Ne)为1.29,多态性信息含量(PIC)为0.16,核苷酸多态性(PI)为0.19,显示马铃薯的遗传多样性水平较低。遗传分化系数、遗传距离、群体进化树和主成分分析显示所有参试品种分为3个类群,遗传分化水平整体较高,但部分品种间的遗传分化系数较低。

The genetic diversity of 24 local potato varieties and one main potato variety were analyzed by genotyping-by-sequencing(GBS)technology in order to explore the level of genetic diversity and genetic differences of local potato varieties in Tibet.The observed heterozygosity(Ho)of 25 potato varieties was 0.24,the expected heterozygosity(He)was 0.19,the effective number of alleles(Ne)was 1.29,the polymorphism information content(PIC)was 0.16,and the nucleotide diversity(PI)was 0.19,indicating that the level of genetic diversity of potatoes was low.The genetic differentiation coefficient,genetic distance,phylogenetic tree,and principal components analysis(PCA)showed that all the varieties were divided into three groups.There were a high level of genetic differentiation,but some varieties were low.