外源蛋白合成抑制剂对杂交水稻种子耐贮藏能力的影响

Effects of exogenous protein synthesis inhibitors on hybrid rice seed storability

以不耐贮藏杂交水稻II优998种子及博优998(对照)种子为材料,采用iTRAQ定量蛋白质组学的方法,鉴定2种不同耐贮藏能力种子贮藏前后的差异蛋白,共鉴定出5210个蛋白,其中差异显著的蛋白100个;II优998种子在贮藏期间的差异蛋白数量显著高于对照种子的差异蛋白数;通过Pathway显著性富集的方法,确定差异蛋白参与的主要代谢途径是Ribosome途径;II优998种子在自然条件贮藏期间有大量的核糖体蛋白变化差异,表明蛋白质生物合成可能与种子耐贮藏能力有关。灌浆期利用3种外源蛋白合成抑制剂(农用链霉素、春雷霉素和广谱性农药咪鲜胺)喷施种子,结果表明,喷施农用链霉素可以提高杂交水稻种子自然条件下的耐贮藏能力。

In this study,a quantitative proteomics of iTRAQ was conducted for rice seeds before and after 24-month natural storage on two hybrid rice cultivars,II You 998 with low storability and the control Boyou 998.More than 5210 proteins were identified,of which 100 differentially expressed protein(DEP)were significant different.Interestingly,the amount of differentially expressed proteins in the storage period of the seeds with poor storability was significantly higher than that of the control seeds.Furthermore,ribosome pathway was determined to be the main metabolic pathway involved in regulation of seed storability by pathway significant enrichment.Our data showed that more ribosomal proteins were synthesized in the seeds which were not resistant to storage,suggesting that protein biosynthesis might be related to the storage tolerance of seeds.And,the germination percentages of hybrid rice seeds treated with different exogenous protein synthesis inhibitors during the filling stage significantly varied after 2 years storage under natural conditions.Our study showed that treatment of agro-streptomycin,during the filling stage could effectively enhance rice seed storability under natural storage conditions.