二十二碳六烯酸联合顺铂对胃癌细胞MGC-803增殖及凋亡的影响

Effects of docosahexaenoic acid combined with cisplatin on proliferation and apoptosis of gastric cancer cell MGC-803

目的研究二十二碳六烯酸(DHA)联合顺铂(DDP)对人胃癌MGC-803细胞增殖及凋亡的影响,并探讨机制。方法MTT检测DHA联合DDP对MGC-803细胞增殖影响,计算IC50及Q值;DAPI染色观察细胞凋亡及形态;FCM检测细胞周期及凋亡率;细胞免疫组化法检测survivin、NF-κB蛋白的表达。结果DHA对MGC-803细胞增殖呈时间剂量依赖性抑制作用,联合DDP后增殖抑制作用变强。Q>0.85,DHA协同增强DDP作用;DAPI染色后观察到细胞凋亡;DHA联合DDP较单药组凋亡率升高(P<0.05);联合组G0/G1期细胞增多(P<0.01),G2/M及S期细胞减少更明显(P=0.01)。联合组survivin、NF-κB蛋白表达水平低于单药组(P<0.05)。结论DHA可协同抑制MGC803细胞体外增殖,可能与调控细胞周期及下调survivin、NF-κB蛋白的表达,诱导细胞凋亡、提高化疗敏感性有关。

Objective Study the effects of docosahexaenoic acid combined with cisplatin on the proliferation and apoptosis of human gastric cancer MGC-803 cells,to analyze its possible mechanisms.Methods MTT evaluate the repression effect of DHA combined with DDP on MGC803 cells.calculate the IC50 and Q values.Observed apoptosis and morphology by DAPI,The cell cycle distribution and apoptosis rate were examined by flow cytometry.The expressions of survivin/NF-κB proteins were detected by immunohistochemical method.Results The proliferation of MGC803 cells was inhibited by DHA in a time-dose dependent manner.The inhibitory effect of combined group on the proliferation of MGC-803 cells were more obvious.Q>0.85,The DHA strengthen the cytotoxic effect of DDP on MGC803 cells.Drug treatment showed characteristics of apoptosis by using fluorescence microscopy.The apoptotic rate of DHA group,the apoptotic rate of combination group was higher than single drug group(P<0.05).The number of the combination group cells in G0/G1 phase increased(P<0.01),the number of G2/M and S phase cells decreased(P=0.01).The expression levels of survivin and NF-κB protein in combined group were lower than single drug group(P<0.05).Conclusion DHA can synergistic inhibit the proliferation of MGC803 cells,related to the regulation of cell cycle,down regulation of survivin and NF-κB protein expression,induction of apoptosis and enhance-ment of chemosensitivity.