碳青霉烯类耐药肠杆菌科细菌的检测方法

Determination methods for carbapenem-resistant Enterobacteriaceae

由碳青霉烯类耐药肠杆菌科细菌(CRE)引起的感染给临床治疗带来了巨大挑战。及时、准确地鉴别出产何种碳青霉烯酶,对指导临床抗感染治疗有至关重要的意义。目前,碳青霉烯酶的检测方法主要为表型筛选、基因检测和基于基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)技术的检测方法。文章对CRE的各类检测方法进行综述。

The infection caused by carbapenem-resistant Enterobacteriaceae(CRE)brings a challenge for clinical treatment,and timely and accurately identifying carbapenemase plays a role in guiding clinical antibiotic application.At present,the determination methods of carbapenemase include phenotypic screening,gene determination and the methods based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS).This review focuses on various determination methods for CRE.