苯甲酸雌二醇诱导小鼠胸腺萎缩模型研究

Study on the Mouse Model of Thymus Atrophy Induced by Estradiol Benzoate

目的对比研究苯甲酸雌二醇(以下简称"雌二醇")不同造模给药次数诱导的小鼠胸腺萎缩模型的差异。方法将44只ICR小鼠平均分为4组,隔天注射雌二醇注射液(每次0.1 mg),其中雌二醇A组共注射3次,雌二醇B组共注射6次,雌二醇C组共注射9次,对照组不做任何实验操作。末次给药24 h后检测其胸腺和脾脏指数、血液指标、胸腺病理变化、胸腺细胞的凋亡以及相关蛋白的表达情况。结果雌二醇A、B组小鼠胸腺指数与对照组相比均显著降低(P<0.01);雌二醇B组的白细胞计数高于对照组(P<0.05),雌二醇A、B组的淋巴细胞百分率较对照组降低(P<0.01);雌二醇B、C组血浆中MDA的含量与对照组相比均显著升高(P<0.01),A组血浆GST的含量较对照组升高(P<0.05);HE染色与TUNEL凋亡检测结果表明雌二醇B组胸腺病变最严重,细胞大量凋亡,而雌二醇C组已与对照组接近。雌二醇B组胸腺凋亡及炎症蛋白(caspase-3、NF-?B)大量激活,远高于其他组别(P<0.01)。结论造模给药3次与6次的雌二醇模型较为理想,而增加造模给药次数不能使其维持模型状态。

OBJECTIVE To compare the differences of mouse thymus atrophy models induced by different administration times of estradiol benzoate(herein after reffered to as "estradiol"). METHODS Forty-four mice were divided into 4 groups on average. Estradiol benzoate injection(0.1 mg each) was injected every other day, in which estradiol A group was injected 3 times, estradiol B group was injected 6 times, and estradiol C group was injected 9 times. The control group did not perform any experimental operation. The thymus and spleen index, blood index, pathological changes of thymus, apoptosis of thymic cells and expression of related proteins were measured 24 h after the last administration. RESULTS The thymus index of mice in estradiol A and B group was significantly reduced compared with control group(P<0.01). In the blood cell analysis results, the white blood cell count of the estradiol B group was higher than that of the control group(P<0.05), and the percentage of lymphocytes of the A and B groups was lower than the control group(P<0.01). The content of MDA in the plasma of estradiol B and C group was significantly increased compared with control group(P<0.01), and the content of GST in the plasma of estradiol A group was higher than that in the control group(P<0.05). The results of HE staining and TUNEL apoptotic examination showed that the thymus lesions in the estradiol B group were the most severe, and the cells had a large number of apoptosis, while the estradiol C group was close to the control group. The thymus apoptosis and inflammatory protein(caspase-3, NF-κB) activation in the estradiol B group were much higher than those in the other groups(P<0.01). CONCLUSION The estradiol model group with 3 and 6 administration times are more successful, and increasing the times of modeling can’t maintain the model state.