阿卡波糖发酵代谢阶段性溶氧控制的研究与应用

Research and Application of Dissolved Oxygen Control in Acarbose Fermentation

目的研究摇瓶发酵不同阶段含氧量变化对阿卡波糖工业生产菌种犹他游动放线菌阿卡波糖合成的影响,以建立溶氧控制策略提高其阿卡波糖发酵水平。方法通过调整摇瓶转速以及降低补料浓度和增加补料量调控溶氧水平,确定发酵过程中溶氧控制对犹他游动放线菌阿卡波糖发酵水平的影响。结果建立了基于提高发酵后期摇床转速并同时降低补料浓度的溶氧控制策略,阿卡波糖效价达到8 307μg·mL^-1,较对照组提升了11.98%。结论通过溶氧控制可以明显提高犹他游动放线菌阿卡波糖发酵水平,结果为进一步提高阿卡波糖工业发酵水平和降低生产成本提供了基础。

OBJECTIVE To establish a dissolved oxygen(DO) control strategy and further improve the acarbose titer, investigate the effect of DO concentration changes in various stages of shake flask fermentation on acarbose synthesis of Actinoplanes utahensis. METHODS By adjusting the rotation speed of shaker, reducing the feed concentration and increasing the feed dosage, the DO concentration was regulated and then the effect of DO control strategy on the acarbose titer by Actinoplanes utahensis was determined. RESULTS A DO control strategy was established by simultaneously increasing the rotation speed and reducing the feed concentration in the later fermentation stage. With the established strategy, the maximum acarbose titer reached 8 307 μg·mL^-1 which was 11.98% higher than that of the control group. CONCLUSION Efficient DO regulation can significantly improve acarbose titer of Actinoplanes utahensis. These findings provide support for further improving the acarbose fermentation and reducing the production costs.