激光解吸后电离质谱成像技术在生物医学分析中的应用

Application of laser desorption post ionization mass spectrometry imaging technology in biomedical analysis

基于激光解吸后电离质谱(LDPI-MS)的分子成像技术是一种新颖的质谱分子成像方法,它已经成为当前质谱技术应用于生物医学领域的热点和前沿技术之一。LDPI是使用两束激光分别完成对样品的解吸和电离任务,这两个过程在时间和空间上都是相互独立的,因此可以单独对每一过程进行优化调节。激光解吸后电离质谱成像技术(LDPI-MSI)通过搭建三维样品移动平台并利用激光对样品进行逐点扫描分析,实现对整个样品内的分子进行成像分析。本文将系统性介绍应用LDPI-MSI的基本原理、主要实验参数优化及其在生物医学分析中的应用。

The molecular imaging technology,which based on laser desorption post ionization mass spectrometry(LDPI-MS)is one of the frontiers and hot technologies in the biomedical field in recent years.LDPI uses two laser beams to complete the desorption and ionization of the sample,respectively.The two processes are independent in time and space,so each process can be optimized and adjusted separately.LDPI-MSI was realized by building a three-dimensional sample stage and scanning the sample dot-by-dot using desorption laser.This paper systematically introduces the basic principle of LDPI-MSI,the optimization of experimental parameters and its application in biomedical analysis.