摘要
由于光学衍射极限的存在,一直以来常规光学显微镜的分辨率被限制在照明光波长的一半左右,这严重限制了生物、材料等研究领域对于更细微结构的观察。共聚焦显微成像技术作为最典型也是最早的点扫描显微技术,凭借其良好的光切片能力和高信噪比成为如今应用最为广泛的光学显微技术。然而由于共聚焦显微成像技术的截止频率有限,分辨率的提升也受到限制。移频技术是将更高频的信息移动到可观察的频率范围,从而提高点扫描显微技术的分辨率。详细介绍了点扫描移频超分辨成像技术的基本原理、优缺点,并对其进行了展望。
The resolution of conventional optical microscopes is limited to about half of the wavelength of illumination light due to the optical diffraction limit,which severely limits the observation of finer structures in biological and material research fields.As the most typical and earliest point scanning microscopy,the confocal microscopy has become the most widely used optical microscopy with good optical slicing ability and high signal-to-noise ratio.However,due to the limited cut-off frequency of the confocal microscopy,the improvement of resolution is also limited.Frequency shifting technique aims to move the higher frequency information to the observable frequency range,so as to improve the resolution of point scanning microscopy.In this review,the basic principle,advantages,and disadvantages of point scanning frequency shifting super-resolution imaging technology are introduced in detail,and its prospect is also given.
作者
陈宇宸
李传康
郝翔
匡翠方
刘旭
Chen Yuchen;Li Chuankang;Hao Xiang;Kuang Cuifang;Liu Xu(State Key Laboratory of Modern Optical Instrumentation,College of Optical Science and Engineering,Zhejiang University,Hangzhou,Zhejiang 310027,China;Collaborative Innovation Center of Extreme Optics,Shanxi University,Taiyuan,Shanxi 030006,China;Ningbo Research Institute,Zhejiang University,Ningbo,Zhejiang 315100,China)
出处
《激光与光电子学进展》
CSCD
北大核心
2020年第18期1-13,共13页
Laser & Optoelectronics Progress
基金
浙江省之江实验室项目(2020MC0AE01)
国家重大科研仪器研制项目(61827825)。
关键词
显微
点扫描
超分辨成像
移频
非线性
探测器阵列
microscopy
point scanning
super-resolution imaging
frequency shifting
nonlinearity
detector array
