MiR-23a靶向HOXC8在黑龙江省宫颈癌人群中发生的作用分析

Analysis of the Role of MiR-23a Targeting HOXC8 in Patients with Cervical Cancer in Heilongjiang Province

目的:研究Mi R-23a靶向HOXC8在黑龙江省宫颈癌人群中发生的作用及机制。方法:采集从2017年1月~2019年1月,于我院确诊为正常宫颈、宫颈上皮内瘤变(CIN)、宫颈癌患者的病变组织各90例。采用逆转录多聚酶联反应(RT-PCR)法分别检测正常宫颈组织、CIN组织、宫颈癌组织中的Mi R-23a表达水平。此外,通过■转染试剂将Mi R-23a模拟物、Mi R-23a抑制物以及空白对照片段转染至宫颈癌细胞Siha中,检测转染后0 h、48 h、72 h时三组Siha细胞的增殖能力情况。另外,以RT-PCR法检测正常宫颈组织、CIN组织、宫颈癌组织中的HOXC8表达水平。结果:宫颈癌组织中Mi R-23a的相对表达量显著高于CIN组织与正常组织,且CIN组织中Mi R-23a的相对表达量显著高于正常组织(均P<0.05)。Mi R-23a模拟物组Siha细胞的增殖能力显著优于Mi R-23a抑制物组与空白对照组,且空白对照组Siha细胞的增殖能力明显优于Mi R-23a抑制物组(均P<0.05)。宫颈癌组织中HOXC8的相对表达量显著高于CIN组织与正常组织,且CIN组织中HOXC8的相对表达量显著高于正常组织(均P<0.05)。结论:Mi R-23a可能是通过靶向下调HOXC8基因的表达,进一步促进了宫颈癌的发生、发展,这为临床宫颈癌的防治提供了新的靶点,值得临床重点关注。

Objective:To study the role and mechanism of Mi R-23a targeting HOXC8 in cervical cancer population in heilongjiang province.Methods:From January 2017 to January 2019,90 patients with normal cervical,cervical intraepithelial neoplasia(CIN)and cervical cancer were collected from our hospital.The expression levels of Mi R-23a in normal cervical tissues,CIN tissues and cervical cancer tissues were detected by RT-PCR.In addition,■transfection reagent was used to transfect Mi R-23a mimics,Mi R-23a inhibitors and blank control fragments into cervical cancer cell Siha,and the proliferation capacity of the three groups of Siha cells was detected at 0h,48h and 72h after transfection.In addition,the expression levels of HOXC8 in normal cervical tissues,CIN tissues and cervical cancer tissues were detected by RT-PCR.Results:The relative expression of Mi R-23a in cervical cancer tissue was significantly higher than that in CIN tissue and normal tissue,and the relative expression of Mi R-23a in CIN tissue was significantly higher than that in normal tissue(all P<0.05).The proliferation ability of Siha cells in the Mi R-23a simulant group was significantly better than that in the Mi R-23a inhibitor group and the blank control group,and the proliferation ability of Siha cells in the blank control group was significantly better than that in the Mi R-23a inhibitor group(all P<0.05).The relative expression of HOXC8 in cervical cancer tissue was significantly higher than that in CIN tissue and normal tissue,and the relative expression of HOXC8 in CIN tissue was significantly higher than that in normal tissue(all P<0.05).Conclusion:Mi R-23a may further promote the occurrence and development of cervical cancer by targeting and down-regulating the expression of HOXC8 gene,which provides a new target for the prevention and treatment of clinical cervical cancer and deserves clinical focus.