不同处理方法对西番莲种子萌发的影响

Effect of different treatment methods on the germination of Passiflora caerulea seeds

[目的]探究打破西番莲种子休眠以促进种子萌发的最适培养基及萌发方式,加快其实生苗的繁育.[方法]以黄果西番莲种子为试验材料,研究不同培养基(1/2MS、3/4MS、MS和PNM)、不同剥壳方式(不剥、只裂不剥、半剥和全剥)及不同质量浓度(0、5、10、15、20 mg􀅰mL^-1)赤霉素(GA3)浸种对西番莲种子萌发率和萌发速率的影响.[结果]4种剥壳处理中,西番莲种子全剥壳处理萌发率最高,为73.63%;GA3浸种中,以5 mg􀅰mL^-1GA3浸种萌发率(82.22%)最高,GA3浓度过高则抑制种子萌发;4种培养基中,以PNM培养基萌发率最高(68.89%),显著高于其他培养基.[结论]西番莲种子在5 mg􀅰mL^-1 GA3浸种12 h后全剥壳接种于PNM培养基,是其萌发的最佳方法,萌发率达到82.22%.该法有利于西番莲实生苗的快速繁殖.

[Purpose]To explore the best culture medium and the way of germination for breaking the seed dormancy of Passiflora caerulea seeds and accelerating the propagation of seedlings.[Method]The seeds of P.caerulea were used as experimental material.The effects of different culture media(1/2 MS,3/4 MS,MS and PNM),different deshelling methods(no peeling,just split,semi-peeling,full peeling),and different concentrations(0,5,10,15,20 mg􀅰mL^-1)of GA3 on the germination rate and germi-nation rate of P.caerulea seeds were studied.[Result]Among the deshelling methods,the germination rate of fully peeled P.caer-ulea seeds was the highest at 73.63%.The highest seed germination rate(82.22%)was observed after soaking in 5 mg􀅰mL^-1 GA3,but the germination was somehow inhibited when the GA3 concentration was too high.Among the culture media tested,the PNM media produced the highest seed germination rate of 68.89%,which was significantly higher than the rates from the other media(P<0.01).[Conclusion]The best method for germinating P.caerulea seeds was pre-soaking the seeds in 5 mg􀅰mL^-1 GA3 for 12 hours,followed by full peeling and inoculating on PNM medium.The germination rate reached 82.22%.This method was beneficial to the rapid propagation of P.caerulea seedlings.