鸦胆子油乳对人白血病多药耐药K562/VCR细胞株增殖抑制的作用探讨

Effect of Brucea javanica oil emulsion on proliferation inhibition of multi-drug resistant K562/VCR cell line of human leukemia

目的:探讨鸦胆子油乳对人白血病多药耐药K562/VCR细胞株增殖抑制的作用。方法:使用四唑盐(MTT)比色法对经过鸦胆子油乳干预作用后的体外细胞K562/VCR细胞增殖抑制比率进行检测。结果:随着鸦胆子油乳浓度水平的不断升高(125、250、500、750、1000 mg/L),对K562细胞的抑制比率持续增加,差异有统计学意义(P<0.05)。随着鸦胆子油乳作用时间的不断增加(24、48、72 h),对K562细胞的抑制比率持续增加,差异有统计学意义(P<0.05)。随着鸦胆子油乳浓度水平的不断升高(500、750、1000、1500、2000 mg/L),对K562/VCR细胞的抑制比率持续增加,差异有统计学意义(P<0.05);随着鸦胆子油乳作用时间的不断增加(24、48、72 h),对K562/VCR细胞的抑制比率持续增加,差异有统计学意义(P<0.05)。伴随鸦胆子油乳浓度水平的不断升高(空白对照组、鸦胆子油组500、750、1000、1500 mg/L),K562/VCR细胞周期当中的G 0/G 1比率持续升高,S比率及G 2/M比值持续降低,差异有统计学意义(P<0.05)。注射鸦胆子油乳之后,K562/VCR细胞当中的ADM浓度(荧光强度)显著升高(161.4±10.9比95.9±8.1),差异有统计学意义(P<0.05)。结论:鸦胆子油乳能够对多药耐药K562/VCR细胞株增殖产生抑制作用,同时还能够对多药耐药细胞周期的阻滞产生诱导作用;鸦胆子油乳能够增加多药耐药K562/VCR细胞株当中化疗药物的积贮量。

Objective To investigate the inhibitory effect of Brucea javanica oil emulsion on the proliferation of human leukemia multi-drug resistant K562/VCR cell line.Methods Methyl thiazolyl tetrazolim(MTT)colorimetric method was used to detect the proliferation inhibition rate of K562/VCR cells in vitro after intervention with Brucea javanica oil emulsion.Results With the increasing concentration of Brucea javanica oil emulsion(125,250,500,750,1000 mg/L),the inhibition ratio to K562 cells continued to increase(P<0.05),and with the increasing duration of Brucea javanica oil emulsion(24,48,72 h),the inhibition ratio to K562 cells continued to increase(P<0.05).With the increasing concentration of Brucea javanica oil emulsion(500,750,1000,1500,2000 mg/L),the inhibition ratio of K562/VCR cells continued to increase(P<0.05),and with the increasing action time of Brucea javanica oil emulsion(24,48,72 h),the inhibition ratio of K562/VCR cells continued to increase(P<0.05).With the increasing concentration of Brucea javanica oil emulsion(blank control group,Brucea javanica oil emulsion group for 500,750,1000,1500 mg/L),the G0/G1 ratio in K562/VCR cell cycle continued to increase,while the S ratio and G2/M ratio continued to decrease(P<0.05).After injection of Brucea javanica oil emulsion,ADM concentration(fluorescence intensity)in the cells increased significantly(161.4±10.9 vs.95.9±8.1)(P<0.05).Conclusions Brucea javanica oil emulsion can inhibit the proliferation of multi-drug resistant K562/VCR cell lines and induce the arrest of multi-drug resistant cell cycle.Brucea javanica oil emulsion increasesd the accumulation of chemotherapeutic agents in multi-drug resistant K562/VCR cell lines.