亚麻荠油脂相关转录因子CsLEC2基因家族的鉴定及表达分析

Identification and Expression Analysis of Oil-Related Transcription Factor CsLEC2 Gene Family in Camelina sativa(L.) Crantz

对亚麻荠CsLEC2家族进行全基因组鉴定、半定量RT-PCR、qRT-PCR,检测CsLEC2基因的时空表达特性并通过转录组数据预测CsLEC2下游靶基因,以解析CsLEC2调控亚麻荠种子油脂合成和积累等生物学功能。结果表明,在亚麻荠基因组中共鉴定到3个亚麻荠CsLEC2基因(CsLEC2.1、CsLEC2.2和CsLEC2.3)。蛋白理化性质和高级结构分析显示,亚麻荠CsLEC2蛋白具有与拟南芥AtLEC2相似的理化性质,且其二级结构的主体也相似。系统进化分析表明,亚麻荠CsLEC2蛋白与模式植物拟南芥AtLEC2蛋白及拟南芥琴亚种AlLEC2蛋白亲缘关系最近。qRT-PCR结果显示亚麻荠CsLEC2在未成熟的种子中高表达。转录组数据分析显示下游基因CsWRI1和CsOLE3高表达,推测CsLEC2可能直接上调CsWRI1和CsOLE3的转录表达,参与油脂代谢调控。

We conducted the genome-wide identification of CsLEC2 gene family and examined spatiotemporal expression patterns of CsLEC2 genes and their downstream target genes by semi-quantitative RT-PCR and qRT-PCR, predicted the downstream target genes of CsLEC2 using transcriptomic data, aiming to analyze the biological functions of CsLEC2 in regulating oil synthesis and accumulation in Camelina sativa seeds. The results showed that CsLEC2.1, CsLEC2.2 and CsLEC2.3 were identified in the genome of C.sativa. Protein physicochemical properties and advanced structural analysis indicated that the C.sativa CsLEC2 protein had similar physicochemical properties and similar secondary structure to Arabidopsis thaliana AtLEC2. Phylogenetic analysis revealed that CsLEC2 protein had the closest genetic relationship with AtLEC2 protein and AlLEC2 protein. qRT-PCR revealed that CsLEC2 only expressed in seeds and had a high expression in immature seeds. The downstream genes CsWRI1 and CsOLE3 were highly expressed, showed CsLEC2 might directly up-regulate the transcriptional expression of CsWRI1 and CsOLE3 involving in oil metabolism regulation.