玉米胚乳中最大量蛋白体的提纯与体外消化

Purification and In-Vitro Digestion of Maximum PBs in Maize Endosperm

为了揭示玉米(Zea mays L.)种质资源醇溶蛋白营养价值的多样性,本研究优化了具有野生型醇溶蛋白性状的玉米自交系PC130胚乳中蛋白体(PBs)的提纯与体外消化环节,结果显示,基于优化后提纯方法的PBs提纯量比已报道方法的获得量提高了50倍以上;通过分析相关因素对最大量PBs提纯物消化的影响,确定0.5mg/mL胃蛋白酶用量与75min处理时间为胚乳PBs的最活跃体外消化条件。

In order to reveal the diversity of nutritional values of zeins in maize germplasm resources, we optimized the extraction and in-vitro digestion of protein bodies(PBs) from the maize inbred line PC130 with the wild type character of zeins. The results showed that the amount of PBs purified with the optimized purification method was more than 50 times higher than the amount based on the reported methods;0.5 mg/mL pepsin-dosage and 75 min treatmenttime were determined as the conditions for the most active in-vitro digestion of endosperm PBs, by analyzing the effects of the relative factors on digestion of the purified maximum-PBs-components.