基于传统煎药工艺的蒲公英饮片标准汤剂制备及质量评价方法研究

Study on Preparation and Quality Evaluation of Standard Decoction of Pugongying(Taraxaci Herba)Based on Traditional Decocting Process

目的建立不同产地13批次蒲公英饮片标准汤剂质量评价方法,并以出膏率、指标成分量及转移率、指纹图谱为指征进行研究。方法以水为溶剂,参照传统煎药工艺制备标准汤剂,采用UPLC-DAD法测定咖啡酸量,计算转移率、出膏率,采用UPLC-ESI-MS/MS法进行指纹图谱研究并确认共有峰。结果13批蒲公英样品制成的标准汤剂中咖啡酸含量为1.9~11.34μg/mL,咖啡酸转移率在4.78%~29.29%,平均转移率为10.65%,标准偏差(RE)为7.06%。出膏率为17.68%~34.25%,平均出膏率为23.36%,标准偏差(RE)为4.99%。指纹图谱共有峰11个,确认6个,分别为绿原酸、咖啡酸、菊苣酸、金丝桃苷、单咖啡酰酒石酸、木犀草苷。结论13批次蒲公英饮片标准汤剂制备工艺符合传统汤剂制法,样品来源具有代表性,样品批次符合指纹图谱研究要求,质量评价内容涵盖面广,所得数据具有代表性,可用于蒲公英饮片标准汤剂的制备及质量评价,可以为配方颗粒标准的制定及工业化生产提供基础数据支撑。

Objective To evaluate the extraction ration,content determination,transfer rationand feature maps or fingerprints by establishing 13 batches of standard decoction of Pugongying(Taraxaci Herba)from different places.Methods Pugongying(Taraxaci Herba)was dissolved in water based onthe traditional decoction process,the content of caffeic acid was determined by UPLC-DAD.The transfer ratio,the extraction ratio,the fingerprints and the common peaks were evaluated by UPLC/Q-TOF-MS/MS.Results The determination of caffeicacid in 13 batches ofstandard decoration was in the range of 1.9~11.34μg/mL,the transfer rate of caffeicacid was in the range of 4.78%~29.29%,the average transfer rate was 10.65%,and the standard deviation was 7.06%.The extraction rate was in the range of 17.68%~34.25%,the average extraction rate was 23.36%and the standard deviation was 4.99%.Eleven peaks of fingerprint were confirmed in this research,and chlorogenic acid,caffeic acid,cichoric acid,hyperoside,caftaric acid and cynaroside were identified.Conclusion The preparation method in this paper conforms to the traditional decoction method,the analyzed samples had representiveness,the batches fit the fingerprint research requirements,the content of quality evaluation covers a wide range and the data obtained are representative.It can be used for the standard preparation and quality evaluation of traditional Chinese medicine and providing basic data for the quality and the industrialization of dispensing granules.