β-榄香烯对CCI模型大鼠疼痛行为学及背根神经节ERK5蛋白的影响

The efects of β-elemene on pain behavior and ERK5 protein in dorsal root ganglia of CCI rats

目的观察β-榄香烯对慢性坐骨神经挤压损伤(CCI)模型大鼠疼痛行为学及背根神经节ERKS蛋白表达的影响。方法SD大鼠32只,建立CCI模型,随机分为造模组、假手术组、文拉法辛组和β-榄香烯组(n=8),对各组大鼠进行机械缩足阈值(PMWT)测定,用免疫组化法测定大鼠背根神经节中ERK5蛋白的表达情况。结果与术前1d比较,术后2d各CCI手术组的PMWT明显降低(P<0.01),说明CCI模型造模成功;与术后2d比较,β-榄香烯组和文拉法辛组术后14~28d的PMWT绝对值明显增加(P<0.05);与模型组比较,β-榄香烯组大鼠背根神经节ERK5的免疫反应积分降低,差异具有显著性(P<0.05)。结论β-榄香烯可上调CCI模型大鼠机械痛阈值,并且抑制模型大鼠背根神经节ERK5蛋白的表达从而对神经病理性疼痛起到干预作用。

Objective To investigate the effect ofβ-elemene on pain behavior and ERK5 Epression of dorsal root ganglion(DRG)in CCI rats.Methods After establishment of CCI models,,thirty-two SD rats were randomly divided into 4 groups(n=8):sham group,model group,venlafaxine group andβ-elemene group.Each rat was tested with PMWT,and ERK5 albumen expression in DRG were detected by immunohistochemistry assay.Results Compared to 1d before CCI operation,the thresholds of PMWT of every CCI operation group were significantly decreased at 2d after CCI operation(P<0.01),which indicated the successful establishment of CCI rats model.Compared to 2d after CCI operation,the PMWT thresholds were significantly increased from 14d to 28d after CCI operation inβ-elemene group and venlafaxine group(P<0.05)Compared with the model group,the integral of immune response of ERKS albumen ofDRG inβ-elemene group was significantly reduced(P<0.05).Conclusionβ-elemene can intervene neuropathic pain by up-regulating the PMWT thresholds and reducing DRG BDNF expression in CCI rats.