黑龙江省高寒地区微藻的分离鉴定以及能源藻株的筛选

Isolation and Identification of Microalgae and Screening of Energy Algae Species in Alpine Region of Heilongjiang Province

为了获得油脂高产的优良藻株,探究不同藻株的生长情况及产油情况。本研究以黑龙江省大庆地区湖泊作为研究对象采集水样,共分离筛选获得8株藻株,通过形态学与分子生物学手段对藻株进行种属鉴定;同时利用RDA分析藻株种类与采样湖泊理化因素之间的关系;对藻株的生长情况、脂质合成以及副产物含量进行了研究。结果表明HDL01、HDL04、HDL05和HDL07的生物量与某些环境因子之间呈正相关。单针藻(Monoraphidium convolutum)HDL08的生物量增长率和脂质增长率仅次于HDL01,为0.077±0.006 g/(L·d)和5.994±0.003 mg/(L·d)。结合微藻的脂质组成、副产物含量分析,发现HDL08有作为生产生物柴油供试藻株的潜力,这对丰富中国藻种资源和利用微藻生产生物柴油提供一定的支撑。

The objectives are to obtain an excellent strain of algae with high oil production,and explore the growth and oil production of different algae strains.In this study,water samples were collected from lakes in Daqing area of Heilongjiang Province.A total of 8 algae strains were isolated and screened.Species identification of algal strains was carried out by morphological and molecular biological methods.At the same time,the relationship between the algae species and the physical and chemical factors of the lake was analyzed by RDA.The growth,lipid synthesis and by-product content of the algae strain were studied.The results showed that the biomass of HDL01,HDL04,HDL05 and HDL07 were positively correlated with some environmental factors.The biomass growth rate and lipid growth rate of Monoraphidium convolutum HDL08 were second only to that of HDL01,which was 0.077±0.006 g/(L·d)and 5.994±0.003 mg/(L·d),respectively.Combined with the analysis of lipid composition and by-product content of algal strains,it was found that HDL08 had the potential to produce biodiesel for the test algae strain,which provided a certain support for enriching Chinese algae resources and using microalgae to produce biodiesel.