哈蟆油与蟾蜍油蛋白质组数据分析

Oviductus Ranae and Oviducts of Bufo Gargarizans:Proteomics Analysis

旨在筛选哈蟆油和蟾蜍油中特异性的蛋白质,发现这些差异蛋白与生长环境、冬眠及发育的关系。采用i TRAQ结合质谱技术建立哈蟆油和蟾蜍油蛋白质组数据库,通过比较两个蛋白质组数据库,对筛选的差异蛋白进行Gene ontology功能注释和生物信息学分析。结果表明,差异表达蛋白的总数为68个,其中上调蛋白61个,下调蛋白7个。哈蟆油中含量较高的7个差异蛋白主要分为五大类,黏蛋白类(PREDICTED:mucin-5AC-like,PREDICTED:similar to mucin,mCG142254, partial)、胶原蛋白(collagen alpha-1(Ⅲ) chain)、α激酶锚定蛋白13样(PREDICTED:a-kinase anchor protein 13-like)、免疫球蛋白Fc段连接蛋白样(PREDICTED:IgGFc-binding protein-like)、膜联蛋白(annexin A1),以上蛋白主要参与免疫调节、生长发育等过程;同时发现哈蟆油中抗冻成分主要为黏蛋白类,而蟾蜍油中主要为凝集素。哈蟆油中含量较高的7个差异蛋白质可作为哈蟆油区别于蟾蜍油的重要成分,同时哈蟆油的特异性膨胀度可能与含量较高的黏蛋白类密切相关。

The aim is to select differentially expressed proteins in oviducts of Chinese Brown frog(OR) and Bufo gargarizans(OBG), and then to find the relationship between these differentially expressed proteins and growth environment, hibernation as well as developments. The proteomics database of OR and OBG were established using iTRAQ technology combined with MS. By comparing the two proteomics database, GO annotation and bioinformatics analysis was conducted for the selected proteins. The results showed that there were 68 differential proteins, including 61 up-regulated proteins and 7 down-regulated proteins. In OR, 7 high expressed proteins were divided into 5 categories, mucins(PREDICTED: mucin-5 AC-like, PREDICTED:similar to mucin, mCG142254, partial), collagen alpha-1(Ⅲ) chain, PREDICTED: a-kinase anchor protein 13-like, PREDICTED: IgGFc-binding protein-like, and annexin A1, which mainly participated in immunomodulation and development. At same time, antifreeze ingredients were mainly mucins in OR, however which were lectin in OBG. 7 high expressed proteins were important proteins in OR, which could be used to distinguish OBG from OR. Exceptional swelling capacity of OR was closely related to a large amount of mucins.