LncRNA XLOC_109948在急性髓系白血病患者中的表达变化及临床意义

Expression and Clinical Significance of LcnRNA XLOC_109948 in Patients with Acute Myeloid Leukemia

目的:检测急性髓系白血病(AML)患者骨髓和血清中LncRNA XLOC_109948的表达水平并验证二者表达的一致性。同时探讨LncRNA XLOC_109948表达变化在AML发生、发展中的临床意义。方法:收集62例AML患者的骨髓组织和静脉血样本,其中36例为AML患者,26例为完全缓解的AML患者(AML-CR组)。同时收集20例健康体检者的静脉血,设为对照组。采用实时荧光定量PCR(qRT-PCR)检测LncRNA XLOC_109948表达水平,分析其表达水平与临床特征的关系。结果:AML组患者骨髓及血清中LncRNA XLOC_109948表达量均高于AML-CR组和对照组(P<0.001)。而其在AML-CR组和对照组2者之间表达差异无统计学意义(P>0.05)。LncRNA XLOC_109948的表达水平在化疗缓解时明显下调,复发时明显上调,且均具有统计学意义(P<0.05)。LncRNA XLOC_109948的表达与细胞遗传学临床病理参数显著相关(P<0.05),但与性别、年龄、WBC计数、骨髓blast、FAB亚型等临床特征无相关性(P>0.05)。结论:AML患者骨髓和血清中LncRNA XLOC_109948呈高表达,且两者表达时序性一致。其表达变化可反映AML发生发展、化疗效果及预后的判断。

Objective:To detect the expression level of LncRNA XLOC_109948 in bone marrow and serum of patients with acute myeloid Leukemia(AML),to verify the consistency between the expression in bone marrow and serum and to explore the role of LncRNA XLOC_109948 expression in the occurrence a development of AML.Methods:Bone marrow and peripheral blood samples were collected from 62 patients with AML,including 36 patients with AML(AML group),26 AML patients with complete remission(AML-CR group),and peripheral blood from 20 healthy persons(control group)were also collected.The expression level of LncRNA XLOC_109948 was detected by real-time quantitative fluorescence PCR(qRT-PCR),and the relationship between its expression and clinical characteristics was analyzed.Results:The expression of LncRNA XLOC_109948 in bone marrow and serum of AML patients was higher than that of AML patients with complete remission and healthy people(P<0.001).And there was no statistically significant difference between the AML-CR group and control group(P>0.05).The expression of Lnc RNA XLOC_109948 significantly decreased when AML patients reached to CR,and significantly increased when the disease relapsed(P<0.05).The expression of LncRNA XLOC_109948 significantly correlated with the clinicopathologic parameters of cytogenetics(P<0.05),but not significantly correlated with sex,age,WBC count,blast in bone marrow,FAB classification and other clinical characteristics(P>0.05).Conclusion:The expression of LncRNA XLOC_109948 in bone marrow and serum of AML patients is high,and its expression in time and sequence is consistent between bone marrow and serum,which can reflect the occurrence,development,chemotherapy efficacy and prognosis of AML patients.