基于浙江北部及上海天蛾科昆虫COI基因的分类系统研究

Preliminary Study on Classification System Based on COI Genes of Sphingidae from Northern Zhejiang and Shanghai

采集并测序了浙江北部及上海市天蛾科Sphingidae昆虫31种94个个体的COI基因片段,构建了系统发育树。研究结果显示,天蛾科是个单系群,同种同属全部各自聚在一起,后验概率全部>95%(平背天蛾Cechetra minor除外);天蛾科分为2大支A和B。A支包括六点天蛾属Marumba,鬼脸天蛾属Acherontia,大背天蛾属Meganoton,白薯天蛾属Agrius,霜天蛾属Psilogramma,锯翅天蛾属Langia,星天蛾属Dolbina,鹰翅天蛾属Ambulyx,豆天蛾属Clanis,构月天蛾属Parum,锤天蛾属Neogurelca,盾天蛾属Phyllosphingia,其中,大背天蛾属、白薯天蛾属、霜天蛾属的亲缘关系较近,其余属的相互关系不明确。B支分为2小支,一支包括长喙天蛾属Macroglossum,缺角天蛾属Acosmeryx;另一支包括葡萄天蛾属Ampelophaga,灰天蛾属Acosmerycoides,背线天蛾属Cechetra,斜纹天蛾属Theretra,白肩天蛾属Rhagastis,红天蛾属Deilephila。Kawahara和朱弘复等提出的两种分类系统各有利弊,有部分类群表现为非单系群,不能很好地与本研究结果相吻合。后续研究应采用多基因建树,或者应用系统发育基因组学对天蛾科的演化关系进行更为深入的研究。

In 2016,June to August 2019,94 individuals of 31 species of Sphingidae were collected form the northern Zhejiang and Shanghai for sequencing their COI.The result indicated that Sphingidae was monophyletic group.The analysis demonstrated that the family of Sphingidae could be divided into 2 subgroups.Comparisons were made on classification systems by Kawahara et al and Zhu et al.