eIF4A1表达与胃癌发生发展及对胃癌细胞侵袭能力的影响

Expression of eIF4A1 and the development of gastric cancer and its influence on the invasion of gastric cancer cells

目的探讨真核起始因子4A1(eIF4A1)在胃癌组织中的表达及对胃癌细胞侵袭、增殖的影响。方法选取2016年1月至2019年12月我院保存的胃癌组织标本190例,同时选取癌旁正常组织作为对照,采用免疫组化染色法检测eIF4A1表达;选取人胃癌细胞株AGS和SGC7901,将细胞随机分为对照组、阴性对照组和干预组,其中对照组加细胞培养液,阴性对照组加含0.5%DMSO的细胞培养液,干预组加eIF4A1表达抑制剂Silvestrol(在DMSO中溶解),采用MTT法检测细胞增殖,Transwell检测细胞侵袭能力。结果胃癌组织eIF4A1阳性表达率为81.05%,明显高于癌旁组织16.84%(P<0.05)。TNM分期Ⅲ~Ⅳ期、有淋巴结转移胃癌组织eIF4A1阳性表达率分别为92.31%和92.78%,明显高于Ⅰ~Ⅱ期、无淋巴结转移胃癌组织(P<0.05)。在AGS细胞试验中,干预组培养12 h、24 h和48 h时的OD值分别为0.312±0.101、0.511±0.106和0.663±0.109,明显低于对照组和阴性对照组(P均<0.05);在SGC7901细胞试验中,干预组培养12 h、24 h和48 h时的OD值分别为0.355±0.103、0.542±0.109和0.700±0.101,明显低于对照组和阴性对照组(P均<0.05)。在AGS细胞试验中,干预组细胞侵袭数为(12.03±3.11)个,明显低于对照组和阴性对照组(P均<0.05);在SGC7901细胞试验中,干预组细胞侵袭数为(57.64±10.11)个,明显低于对照组和阴性对照组(P均<0.05)。结论胃癌组织中eIF4A1呈高表达,与临床分期、淋巴结转移有一定相关性;抑制eIF4A1表达,对胃癌细胞增殖、侵袭有一定抑制作用。

Objective To investigate the expression of eukaryotic initiation factor 4 A1(e IF4 A1)in gastric cancer and its effect on the invasion and proliferation of gastric cancer cells.Methods From January 2016 to December 2019,190 specimens of gastric cancer were collected from our hospital,the adjacent tissues were selected as the control,and the expression of e IF4 A1 was detected by immunohistochemistry;Human gastric cancer cell lines AGS and SGC7901 were selected,the cells were randomly divided into control group,negative control group and intervention group,the control group was added with cell culture medium,0.5%DMSO was added in the negative control group,e IF4 A1 expression inhibitor Silvestrol(dissolved in DMSO)was added in the intervention group,MTT method was used to detect cell proliferation,Transwell was used to detect cell invasion.Results The positive expression rate of e IF4 A1 in gastric cancer was 81.05%which was significantly higher than that in adjacent tissues(P<0.05);The positive rates of e IF4 A1 in TNM stageⅢ~Ⅳand lymph node metastasis gastric cancer were 92.31%and 92.78%which were significantly higher than those in stageⅠ~Ⅱand non lymph node metastasis gastric cancer(P<0.05);In AGS cell test,the OD values of the intervention group at 12 h,24 h and 48 h after cultured were 0.312±0.101,0.511±0.106 and 0.663±0.109,which were significantly lower than those of the control group and the negative control group(P<0.05);In SGC7901 cell test,the OD values of the intervention group at 12 h,24 h and 48 h after cultured were 0.355±0.103,0.542±0.109 and 0.700±0.101,which were significantly lower than those of the control group and the negative control group(P<0.05);In AGS cell test,the number of cell invasion in intervention group was(12.03±3.11),which was significantly lower than that in control group and negative control group(P<0.05);In SGC7901 cell test,the number of cell invasion in intervention group was(57.64±10.11)which was significantly lower than that in control group and negative control group(P<0.05).Conclusion The expression of e IF4 A1 is high in gastric cancer,which is correlated with clinical stage and lymph node metastasis.The inhibition of e IF4 A1 expression can inhibit the proliferation and invasion of gastric cancer cells.