风疹疑似样本ELISA与RT-PCR检测结果研究

目的探讨风疹疑似样本和ELISA及RT-PCR测定结果.方法以回顾性分析形式,应用ELISA测定血清样本中的风疹免疫球蛋白抗体,应用RT-PCR测定咽拭子样本中的风疹病毒核糖核酸,同时记录测定结果.结果本次共纳入风疹麻疹疑似血清47份和咽拭子样本13份.47份血清样本经由ELISA测定阳性16份,ELISA阳性率34.04%,实验室确诊21份,确诊率44.68%;ELISA和RT-PCR灵敏度为76.19%和7.69%,经过卡方检验,ELISA阳性率和RT-PCR阳性率对比有差异性(P<0.05).结论早期数据采集较少是导致ELISA阳性率高于RT-PCR的因素,ELISA更适合中后期诊断,RT-PCR适合于早期诊断.

Objective To investigate the possible samples of rubella and the results of ELISA and rt-pcr.Methods Rubella immunoglobulin antibody in serum samples was determined by ELISA,and rubella virus RNA in throat swab samples was determined by rt-pcr.Results A total of 47 suspected rubella measles serum and 13 throat swab samples were included.Sixteen of 47 serum samples were positive by ELISA,with a positive rate of 34.04%by ELISA,and 21 were laboratory confirmed,with a confirmed rate of 44.68%.The sensitivity of ELISA and rt-pcr was 76.19%and 7.69%,and the chi-square test showed that the positive rate of ELISA was different from that of rt-pcr(P<0.05).Conclusion Less early data collection is the factor that leads to higher positive rate of ELISA than rt-pcr.ELISA is more suitable for middle and late diagnosis,while rt-pcr is suitable for early diagnosis.